324761

Elastase Inhibitor V

• Synonym(e): Elastase Inhibitor V, 2-(2-Bromophenyl)-5-chloro-3,1-benzoxazin-4-one
• Empirische Formel (Hill-System): C₁₄H₇BrClNO₂
• Molekulargewicht: 336.57
• NACRES: NA.54
• UNSPSC Code: 12352200
• Assay: ≥99% (HPLC)
• Form: solid
• Quality level: 100
• Storage condition: OK to freeze, desiccated, protect from light

Eigenschaften

• assay: ≥99% (HPLC)
• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, desiccated, protect from light
• color: off-white
• solubility: DMSO: 50 mg/mL (Use only fresh DMSO.)
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

A benzoxazinone compound that acts as a potent inhibitor of human leukocyte elastase (IC₅₀ = 29.5 nM), likely by covalently modifying active site serine via a Michael addition-elimination reaction. Shown to inhibit medium elastase activity (IC₅₀ = 80.8 nM), but not O₂^•- generation, in fLMP (Cat. No. 05-22-2500)-stimulated primary human neutrophil cultures. Reported to reduce neutrophil infiltration in lung after hemorrhagic shock in rats in vivo (1 mg/kg, i.v.) as assessed by increase in lung wet/dry ratio (0.75- vs. 1.23-fold increase, respectively, with or without treatment) and MPO activity (1.08- vs. 3.72-fold increase, respectively, with or without treatment).

Biochem/physiol Actions

Cell permeable: yes

Primary Target
Elastase

Reversible: no

Packaging

Packaged under inert gas

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Hsieh, P.W., et al. 2010. Eur. J. Med. Chem.45, 3111.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable