The concentration of this antibody is 0.06 mg/mL. This information is reported in the lot-specific Certificate of Analysis. This antibody is formulated for use as a component in product 16-202, H2A.X Phosphorylation Assay Kit. This kit also includes an antibody control, product 12-487, Normal Mouse IgG, FITC at a concenration of 50 ug/mL. This information is also reported in the lot-specific Certificate of Analysis.
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
Quality Level
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable, flow cytometry: suitable
NCBI accession no.
UniProt accession no.
detection method
fluorometric
shipped in
dry ice
General description
Das H2A.X-Phosphorylierungs-Assay-Kit (Durchflusszytometrie) ist ein zellbasierter Assay, der für den Durchflusszytometrienachweis von phosphoryliertem Histon H2A.X formatiert wurde. Zellen werden in Mikrotiterplatten kultiviert und mit Mitteln, die DNA-Schäden oder Apoptose einleiten, behandelt, was die H2A.X-Phosphorylierung stimuliert. Die Zellen werden anschließend fixiert und als Vorbereitung für Färbung und Nachweis permeabilisiert. An Serin 139 phosphoryliertes Histon H2A.X wird durch die Zugabe von Anti-Phospho-Histon H2A.X, FITC-Konjugat nachgewiesen. Anschließend werden die Zellen in einem Durchflusszytometer gescannt, um die Anzahl von Zellen, die positiv für phosphoryliertes Histon H2A.X färben, zu quantifizieren.
Application
Neurodegenerative Erkrankungen
Neurowissenschaft
Packaging
Analysis Note
Other Notes
Normales Maus-IgG, FITC-Konjugat (Katalognr. 12-487)
16X Fixierungslösung
10X Permeabilisierungslösung
10X Waschlösung
Legal Information
Disclaimer
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) activity assay: suitable, flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) immunocytochemistry: suitable | technique(s) ChIP: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable |
| detection method fluorometric | detection method fluorometric | detection method - | detection method - |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
| UniProt accession no. | UniProt accession no. - | UniProt accession no. | UniProt accession no. |
| NCBI accession no. | NCBI accession no. - | NCBI accession no. | NCBI accession no. |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
signalword
Danger
Hazard Classifications
Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Carc. 1B - Eye Dam. 1 - Flam. Liq. 2 - Muta. 2 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1 - STOT SE 3
target_organs
Eyes,Central nervous system, Respiratory system
Lagerklasse
3 - Flammable liquids
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Verwandter Inhalt
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"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
"Recognizing both the tremendous opportunities and the challenges facing cancer research, we are dedicated to developing and refining tools and technologies for the study of cancer. With our comprehensive portfolio, including the Upstate®, Chemicon®, and Calbiochem® brands of reagents and antibodies, researchers can count on dependable, high quality solutions for analyzing all the hallmarks of cancer."
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 17-344 | 04053252582691 |
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