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Rho-Aktivierungs-Assaykit

PP1/PP2A Toolbox for the selective in vitro dephosphorylation of proteins.

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Über diesen Artikel

UNSPSC Code:
12161503
NACRES:
NA.84
eCl@ss:
32161000
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Quality Level

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable, affinity binding assay: suitable

NCBI accession no.

UniProt accession no.

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

dry ice

Gene Information

human ... RHOA(387)

General description

GST-markiertes Fusionsprotein, das den Resten 7–89 der in E. coli exprimierten Rhotekin-Rho-Bindungsdomäne der Maus entspricht. Bei Bereitstellung an Glutathion-Agarose gebunden. Reinheit >90 %.

Application

PP1/PP2A-Toolbox für die selektive In-vitro-Dephosphorylierung von Proteinen.

Packaging

Kit-Kapazität: 30 Assays:

Analysis Note

Regelmäßige Beurteilung durch Ausfällen von GTP-Rho aus 3T3/A31-Zelllysaten und den anschließenden Nachweis mittels Immunoblot-Analyse unter Verwendung von Anti-Rho (05-778).

Other Notes

100X GTPγS, 10 mM (Art.-Nr. 20-176)

100X GDP, 100 mM (Art.-Nr. 20-177)

Mg2+ Lyse-/Waschpuffer, 5X (Art.-Nr. 20-168)

Anti-Rho (-A, -B, -C), Klon 55 (Art.-Nr. 05-778)

Rho-Assay-Reagens (Rhotekin RBD, Agarose) (Art.-Nr. 14-383)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Sofern in unserem Katalog oder anderen Begleitdokumenten unserer Produkte nicht anders angegeben, sind unsere Produkte nur für Forschungszwecke vorgesehen und nicht für andere Zwecke zu verwenden, einschließlich, jedoch nicht beschränkt auf unautorisierte kommerzielle Verwendung, zur In-vitro-Diagnostik, für Ex-vivo- oder In-vivo-Therapiezwecke oder jegliche Art der Einnahme oder Anwendung bei Menschen oder Tieren.

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Dieser Artikel
14-27817-28314-415
technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable, affinity binding assay: suitable

technique(s)

affinity binding assay: suitable

NCBI accession no.

NM_001664.2

NCBI accession no.

NM_001085049.1, NM_012219.3

NCBI accession no.

NM_006908.3, NM_018890.2, NM_198829.1

NCBI accession no.

NM_006788.3

detection method

chemiluminescent, fluorometric, colorimetric

detection method

-

detection method

-

detection method

-

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

Gene Information

human ... RHOA(387)

Gene Information

human ... MRAS(22808)

Gene Information

human ... RAC1(5879)

Gene Information

-

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®


pictograms

CorrosionEnvironment

signalword

Danger

Lagerklasse

10 - Combustible liquids

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1



Analysenzertifikate (COA)

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M P Gratacap et al.
The Journal of biological chemistry, 276(51), 47906-47913 (2001-09-19)
Platelets were used to study the activation of Rho and Rac through G-protein-coupled receptors and its regulation by cyclic nucleotides. The thromboxane A(2) (TXA(2)) mimetic rapidly activated both small GTPases independently of integrin alpha(IIb)beta(3) activation., which leads to the activation
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Global Trade Item Number

SKUGTIN
17-29404053252402814

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