The presence of FBS in the cell culture supernatant containing IgG2b may lead to reduced yield and purity of the mAb, IgG2b, as FBS contains bovine immunoglobulins that can compete with the target antibody for binding to the protein G column.
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
form
liquid
manufacturer/tradename
Upstate®
technique(s)
affinity chromatography: suitable, immunoprecipitation (IP): suitable, western blot: suitable
shipped in
wet ice
General description
Application
Physical form
Preparation Note
Analysis Note
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| technique(s) affinity chromatography: suitable, western blot: suitable, immunoprecipitation (IP): suitable | technique(s) immunoprecipitation (IP): suitable, western blot: suitable | technique(s) ChIP: suitable | technique(s) - |
| form liquid | form liquid | form liquid | form suspension |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in - |
| manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Upstate® | manufacturer/tradename Roche |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
Lagerklasse
3 - Flammable liquids
wgk
WGK 1
flash_point_f
116.6 °F
flash_point_c
47 °C
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Verwandter Inhalt
Western blotting is one of the most commonly used techniques in the lab, yet difficulties persist in obtaining consistent, quality results. We’ve been helping scientists publish their Western blots for decades, with continued innovation and steadfast technical support. Explore our expanded portfolio of products, including optimized reagents for chemiluminescent and Ḁuorescent Westerns, as well as the SNAP i.d.® system, which reduces blocking, washing and antibody incubation time from hours to minutes.
Protein Blotting Survival Handbook: Tips and Tricks
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 16-266 | 04053252325731 |
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Is the 16-266 protein G column compatible with purifying IgG in the presence of FBS when purifying mAb, IgG2b, from cell culture supernatant containing FBS?
1 answer-
Helpful?
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What is the approximate diameter of each agarose bead?
1 answer-
The diameter range of 4% beaded agarose typically falls between 50 to 165 micrometers.
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