1.50822

LiChrospher^® 100 RP-8 (5 µm) HPLC Columns

L x I.D. 125 mm x 4 mm LiChroCART^™ HPLC cartridge, manuCART^™ holder required for use

• Synonym(e): TM=["LiChrospher"] C8 TM=["LICHROCART"] cartridge for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: C8 (RP8), C8 bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Eigenschaften

• Produktname: LiChrospher^® 100 RP-8 (5 µm) LiChroCART^™ 125-4, suitable for HPLC
• material: stainless steel column
• Quality Segment: 100
• agency: suitable for USP L7
• product line: LiChrospher^®
• feature: endcapped: no
• extent of labeling: 12.5% Carbon loading
• parameter: 250 bar pressure, 60 °C max. temp.
• technique(s): HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 12.5 cm × 4 mm
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: C8 (RP8), C8 bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Beschreibung

General description

HPLC cartridge

LiChrospher^® RP-8 is a reliable and versatile traditionally produced spherical silica gel carrier with C-8 reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 125-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).

Application

• Determination of dopamine, noradrenaline, and adrenaline in Krebs-Henseleit solution by liquid chromatography coupled with tandem mass spectrometry: This study showcases the use of LiChrospher^® 100 RP-8 (5 µm) in a LICHROCART^™ 125-4 setup for precise neurotransmitter measurement in biological samples, advancing understanding in neurochemistry and cardiovascular research. The column′s selectivity and resolution enhance the detection of these small molecules, pivotal for biochemical assays and physiological studies (Britto-Júnior et al., 2021).

Analysis Note

Pressure: ≤ 45 bar
Theoretical Plates (N/m) (Toluene): ≥ 50000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 0.8 - 2.0

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids