This product is the inactive form of the kinase, obtained after purification by Ni-NTA agarose from insect cells. In this initial form after purification, the kinase exhibits negligible activity. No inhibitors or mutations are used to generate the inactive form.
14-279
Akt1/PKBα-Protein, inaktiv, 50 g
Unactive, N-terminal His6-tagged recombinant full-length human Akt1, for use in Kinase Assays.
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
UNSPSC Code:
12352202
NACRES:
NA.26
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 59 kDa
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
human
Quality Level
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 59 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
General description
Am N-Terminus His6-markiertes rekombinantes humanes Akt1 von voller Länge
Analysis Note
regelmäßig mittels Phosphorylierung von Crosstide beurteilt
Other Notes
Spezifische Aktivitätsdaten entnehmen Sie bitte dem Analysenzertifikat, das jeder Charge dieses Enzyms beiliegt.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| recombinant expressed in baculovirus infected Sf21 cells | recombinant expressed in Sf21 cells | recombinant expressed in baculovirus infected Sf21 cells | recombinant expressed in baculovirus infected Sf21 cells |
| biological source human | biological source human | biological source human | biological source human |
| technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (kinase) | technique(s) activity assay: suitable (IP-kinase), activity assay: suitable (kinase) |
| mol wt Mw 59 kDa | mol wt Mw 49.2 kDa | mol wt Mw 45 kDa | mol wt Mw 59.9 kDa |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
Lagerklasse
10 - Combustible liquids
wgk
WGK 2
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Hiroshi Senoo et al.
Nature cell biology, 21(7), 867-878 (2019-07-03)
mTORC2 plays critical roles in metabolism, cell survival and actin cytoskeletal dynamics through the phosphorylation of AKT. Despite its importance to biology and medicine, it is unclear how mTORC2-mediated AKT phosphorylation is controlled. Here, we identify an unforeseen principle by
Jorge Vera et al.
eLife, 4 (2015-11-28)
The PP2A phosphatase is often inactivated in cancer and is considered as a tumour suppressor. A new pathway controlling PP2A activity in mitosis has been recently described. This pathway includes the Greatwall (GWL) kinase and its substrates endosulfines. At mitotic
Dos D Sarbassov et al.
Molecular cell, 22(2), 159-168 (2006-04-11)
The drug rapamycin has important uses in oncology, cardiology, and transplantation medicine, but its clinically relevant molecular effects are not understood. When bound to FKBP12, rapamycin interacts with and inhibits the kinase activity of a multiprotein complex composed of mTOR
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 14-279 | 04053252624872 |
-
Is this prduct a kinase-dead Akt1 mutant?
1 answer-
Helpful?
-