1.16152

LiChrospher^® 100 DIOL (5 µm) HPLC Columns

HPLC sorbent

• Synonym(e): TM=["LiChrospher"] Diol sorbent for HPLC
• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: diol, Diol bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Eigenschaften

• Produktname: LiChrospher^® 100 DIOL (5 µm),
• material: stainless steel column
• agency: suitable for USP L20
• product line: LiChrospher^®
• feature: endcapped: no
• extent of labeling: 8% Carbon loading
• parameter: 60 °C max. temp.
• technique(s): HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: diol, Diol bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• bulk density: 200‑400 kg/m³
• separation technique: hydrophilic interaction (HILIC), normal phase, reversed phase
• storage temp.: no temp limit

Beschreibung

General description

LiChrospher^® DIOL has polar and hydrophobic properties, thus can be used as less polar alternative to LiChrospher Si 60 in normal phase applications or as less hydrophobic alternative to LiChrospher RP-8 in reversed phase applications. LiChrospher DIOL is also suitable for exclusion chromatography.

Application

• Chiral and achiral separation of ten flavanones using supercritical fluid chromatography: This study illustrates the application of LiChrospher 100 DIOL in separating flavanones in bee pollen, utilizing both chiral and achiral approaches. The method offers enhanced accuracy and efficiency for analyzing natural products (Ares et al., 2022).


• Application of Diol column under adsorption and mixed partition-adsorption normal-phase liquid chromatography mode: This research demonstrates the use of LiChrospher 100 DIOL for the separation of purines and pyrimidines, showcasing its versatility in both adsorption and mixed partition-adsorption chromatographic techniques, which are critical for biochemical analysis (Kazoka, 2008).

Analysis Note

Theoretical Plates (N/m) (2-Nitrotoluene): ≥ 50000
stability testing: passes test
Particle Size (d10): 4.8 - 5.5 µm
Particle Size (d50): 6.5 - 7.5 µm
Particle Size (d90): 9.0 - 10.5 µm

Legal Information

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable