1.50960

LiChrospher^® 100 DIOL (5 µm) HPLC Columns

L x I.D. 4 mm x 4 mm LiChroCART^™ HPLC guard-cartridge

• UNSPSC Code: 41115709
• NACRES: SB.52
• Particle size: 5 μm
• Matrix active group: diol, Diol bonding phase
• Pore size: 100 Å pore size
• Matrix: fully porous particle

Eigenschaften

• Produktname: LiChrospher^® 100 DIOL (5 µm) LiChroCART^™ 4-4, suitable for HPLC
• material: stainless steel column
• Quality Level: 100
• agency: suitable for USP L20
• product line: LiChrospher^®
• feature: endcapped: no
• packaging: 10 pak of
• extent of labeling: 8% Carbon loading
• parameter: 250 bar pressure, 60 °C max. temp.
• technique(s): HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
• column L × I.D.: 0.4 cm × 4 mm
• surface area: 350 m²/g
• matrix: fully porous particle
• matrix active group: diol, Diol bonding phase
• particle size: 5 μm
• pore size: 100 Å pore size
• operating pH range: 2-7.5
• separation technique: hydrophilic interaction (HILIC), normal phase, reversed phase
• storage temp.: no temp limit

Beschreibung

General description

HPLC guard column

LiChrospher^® DIOL has polar and hydrophobic properties, thus can be used as less polar alternative to LiChrospher Si 60 in normal phase applications or as less hydrophobic alternative to LiChrospher RP-8 in reversed phase applications. LiChrospher DIOL is also suitable for exclusion chromatography. This sorbent is packed into LiChroCART 4-4 HPLC guard columns, to be used in connection with LiChroCART HPLC cartridges, packed with LiChrospher DIOL, 5µm. The connection is being accomplished by use of the manu-CART NT cartrige holder (1.51486)

Application

• Sensitive determination of a glyoxal-DNA adduct biomarker candidate by column switching capillary liquid chromatography electrospray ionization mass spectrometry.: This study describes the use of LiChrospher^® 100 DIOL (5 µm) in column switching capillary liquid chromatography to determine glyoxal-DNA adducts, which are potential biomarkers for DNA damage. The method combines high sensitivity with electrospray ionization mass spectrometry for precise analysis, highlighting its application in biomarker research and analytical chemistry (Olsen et al., 2008).

Analysis Note

Selectivity: passes test

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany

LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany