07-441
Anti-Dimethyl-Histon-H3(Lys9)-Antikörper
Upstate®, from rabbit
Synonym(e):
H3K9me2, Histone H3 (di methyl K9), H3 histone family, member T, histone 3, H3, histone cluster 3, H3
Größe auswählen
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 μg | Warenkorb auf Verfügbarkeit prüfen | € 583,00 |
Über diesen Artikel
biological source
rabbit
Quality Segment
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, Schizosaccharomyces pombe, rat, chicken
manufacturer/tradename
Upstate®
technique(s)
dot blot: suitable, immunocytochemistry: suitable, inhibition assay: suitable (peptide), western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
dimethylation (Lys9)
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
General description
Immunogen
Application
Spezifizität durch ein unabhängiges Labor bestätigt.
Peptidhemmung:
Die Spezifizität wurde durch die Fähigkeit von 1 μmol/l des immunisierenden Peptids, den Nachweis von Histon H3 in einem Western Blot von HeLa-Säureextrakten vollständig zu beseitigen, bestätigt (Abbildung A, Bahn 4).
Immunzytochemie:
Von einem unabhängigen Labor berichtet.
Biochem/physiol Actions
Physical form
Preparation Note
Handhabungsempfehlungen: Das Fläschchen nach Empfang und vor dem Abnehmen der Kappe zentrifugieren und die Lösung vorsichtig mischen. In Mikrozentrifugenröhrchen aliquotieren und bei -20 °C aufbewahren.
Wiederholte Einfrier-/Auftauzyklen sind zu vermeiden, da sie das IgG beschädigen und die Produktleistung beeinträchtigen können. Hinweis: Veränderungen der Gefrierschranktemperatur unter -20 °C können dazu führen, dass glycerinhaltige Lösungen während der Lagerung einfrieren.
Analysis Note
HELA-Ganzzelllysat, U2OS-Zelllysat.
Western-Blot-Analyse:
Eine 1:500-Verdünnung dieser Charge wies Histon H3 in 10 μg HeLa-Säureextrakt nach, aber nicht in rekombinantem Histon H3.
Other Notes
Legal Information
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| clone polyclonal | clone polyclonal | clone polyclonal | clone polyclonal |
| antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form unpurified | antibody form serum |
| biological source rabbit | biological source rabbit | biological source rabbit | biological source rabbit |
| species reactivity human, mouse, Schizosaccharomyces pombe, rat, chicken | species reactivity Saccharomyces cerevisiae, human, vertebrates, chicken | species reactivity Tetrahymena sp., human | species reactivity human |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
| technique(s) dot blot: suitable, inhibition assay: suitable (peptide), immunocytochemistry: suitable, western blot: suitable | technique(s) immunocytochemistry: suitable, western blot: suitable | technique(s) ChIP: suitable (ChIP-seq), dot blot: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, inhibition assay: suitable (peptide), western blot: suitable | technique(s) dot blot: suitable, inhibition assay: suitable (peptide), western blot: suitable |
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Lagerklasse
10 - Combustible liquids
wgk
WGK 1
Analysenzertifikate (COA)
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Verwandter Inhalt
Cancer is a complex disease manifestation. At its core, it remains a disease of abnormal cellular proliferation and inappropriate gene expression. In the early days, carcinogenesis was viewed simply as resulting from a collection of genetic mutations that altered the gene expression of key oncogenic genes or tumor suppressor genes leading to uncontrolled growth and disease (Virani, S et al 2012). Today, however, research is showing that carcinogenesis results from the successive accumulation of heritable genetic and epigenetic changes. Moreover, the success in how we predict, treat and overcome cancer will likely involve not only understanding the consequences of direct genetic changes that can cause cancer, but also how the epigenetic and environmental changes cause cancer (Johnson C et al 2015; Waldmann T et al 2013). Epigenetics is the study of heritable gene expression as it relates to changes in DNA structure that are not tied to changes in DNA sequence but, instead, are tied to how the nucleic acid material is read or processed via the myriad of protein-protein, protein-nucleic acid, and nucleic acid-nucleic acid interactions that ultimately manifest themselves into a specific expression phenotype (Ngai SC et al 2012, Johnson C et al 2015). This review will discuss some of the principal aspects of epigenetic research and how they relate to our current understanding of carcinogenesis. Because epigenetics affects phenotype and changes in epigenetics are thought to be key to environmental adaptability and thus may in fact be reversed or manipulated, understanding the integration of experimental and epidemiologic science surrounding cancer and its many manifestations should lead to more effective cancer prognostics as well as treatments (Virani S et al 2012).