05-659

Anti-Kaiso Antibody, clone 6F

clone 6F, Upstate^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 6F, monoclonal
• Application: EMSA, IHC, IP, WB
• Citations: 9

Eigenschaften

• Quality Level: 100
• biological source: mouse
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 6F, monoclonal
• species reactivity: mouse, hamster, chicken, canine, rat, human, monkey
• manufacturer/tradename: Upstate^®
• technique(s): electrophoretic mobility shift assay: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG1

Beschreibung

General description

97kDa

Immunogen

Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso

Application

Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Biochem/physiol Actions

Kaiso

Physical form

0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.

Format: Purified

Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1