05-1471

Anti-Rictor Antibody, clone 9F1.2

clone 9F1.2, from mouse

• Synonym(e): Rictor, AVO3, rapamycin-insensitive companion of mTOR, mAVO3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 9F1.2, monoclonal
• Application: IP, WB
• Citations: 6

Eigenschaften

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 9F1.2, monoclonal
• species reactivity: human, mouse, rat
• technique(s): immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_689969.2
• UniProt accession no.: Q6R327
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... RICTOR(253260)

Beschreibung

General description

190 kDa Observed

Rictor, the rapamycin-insensitive complex, is part of the mTORC2 kinase complex which is responsible for phosphorylating the hydrophobic motif kinase of Akt/PKB. This complex also includes mTOR, mSin1 and mLST8/GL. mTORC2 is activated by growth factors and other mitogenic signals.

Immunogen

Recombinant protein corresponding to human Rictor.

Application

Anti-Rictor Antibody, clone 9F1.2 detects level of Rictor & has been published & validated for use in WB & IP.

Immunoprecipitation:
Representative lot data.
1 ug/mL dilution of this antibody immunoprecipitated Rictor from 100 mg of HEK293 lysate.

Biochem/physiol Actions

This antibody recognizes Rictor.

Physical form

Format: Purified

Purified immunoglobulin in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Analysis Note

Control
HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Rictor on 10 µg of HEK293 cell lysate.

Evaluated by Western Blotting on 10 mg of HEK293 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable