03-0184-00

SMC^® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit

• Synonym(e): pTau T181 Single Molecule Counting Kit, pTau T181 immunoassay kit, pTau T181 ultra-sensitive immunoassay, pTau Thr181 immunoassay kit
• UNSPSC-Code: 12161503
• NACRES: NA.84

Eigenschaften

• Hersteller/Markenname: SMC^®
• assay range: sensitivity: 0.94 pg/mL; (Lower Limit of Quantification: ); inter-assay cv: ≤12%; intra-assay cv: ≤7%; (five normal plasma samples run in triplicate by 3 different operators on 3 different days.)
• Methode(n): single molecule counting: suitable
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

The SMC^® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
2) The Erenna and SMCxPRO^® immunoassay systems detect analytes using Single Molecule Counting (SMC^®) technology.

Spezifität

Species Reactivity:
Specificity was not tested.

Cross Reactivity:
Cross-reactivity was not tested.

Accuracy:

• Minimum of 5 serum samples spiked with average recovery of 97% with a range of 70-138%
• Minimum of 5 plasma samples spiked with average recovery of 97% with a range of 81-127%
• Minimum of 5 CSF samples spiked with average recovery of 108% with a range of 82-146%

Anwendung

The sensitivity of the Single Molecule Counting (SMC^®) technology gives the SMC^® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Phospho-Tau (T181) in human serum, plasma, and CSF.

This new streamline kit has the following features including:

• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Removal of a plate transfer step
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO^® and Erenna

Research Category:

• Neuroscience
• Neurological

Research Sub-Category:

• Alzheimer′s Disease
• Aging
• Nervous System
• Neurodegenerative Diseases
• Neurofilament & Neuron Metabolism
• Neuronal & Glial Markers
• Neuroregenerative Medicine
• Neuroscience

Verpackung

96 well assay

Komponenten

• Assay Buffer
• pTau T181 Coated Beads
• Standard Diluent
• pTau T181 Detection Antibody
• pTau T181 Standard
• 10X Wash Buffer
• Buffer D
• Elution Buffer B

Rechtliche Hinweise

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Piktogramme: GHS06,GHS08
• Signalwort: Danger
• H-Sätze: H302 + H332,H311,H319,H360FD,H373,H412
• P-Sätze: P273 - P280 - P301 + P312 - P302 + P352 + P312 - P304 + P340 + P312 - P308 + P313
• Gefahreneinstufungen: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
• Zielorgane: Respiratory Tract
• Lagerklassenschlüssel: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects