03-0184-00

SMC^® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human phospho-Tau (T181) in human serum, plasma, and cerebrospinal fluid (CSF)

• Synonym(e): pTau T181 Single Molecule Counting Kit, pTau T181 immunoassay kit, pTau T181 ultra-sensitive immunoassay, pTau Thr181 immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Eigenschaften

• Produktname: SMC^® Human Phospho-Tau (T181) High Sensitivity Immunoassay Kit,
• Quality Segment: 200
• description: Used for ultrasensitive quantification of Human phospho-Tau (T181) in human serum, plasma, and cerebrospinal fluid (CSF)
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: serum; cerebrospinal fluid (CSF); plasma (K2 EDTA)
• UniProt accession no.: P10636
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Phospho Tau 181 refers to a phosphorylated form of the tau protein, specifically at threonine 181. This post-translational modification influences tau′s structure and function, often leading to its aggregation into neurofibrillary tangles, which are characteristic of various neurodegenerative diseases. In healthy neurons, tau stabilizes microtubules and supports axonal transport. However, when phosphorylated at T181, tau tends to misfold and form aggregates, disrupting microtubule stability and impairing neuronal function.

Elevated levels of phospho Tau 181 in cerebrospinal fluid (CSF) serve as a biomarker for neurodegeneration, particularly in Alzheimer′s disease, where its presence correlates with disease severity and progression. Additionally, phospho Tau 181 contributes to neuroinflammation by activating glial cells, which can exacerbate neuronal damage and promote disease progression.

Application

Alzheimer′s Disease / Aging / Nervous System / Neurodegenerative Diseases / Neurofilament & Neuron Metabolism / Neuronal & Glial Markers / Neurodegenerative Medicine / Neuroscience

Features and Benefits

• Ultrasensitive Detection: Enables femtogram-level measurement of pTau181 in serum, plasma or CSF.
• Enhanced Signal Resolution: Expanded dynamic range with improved signal-to-noise ratio ensures consistent and accurate assay results.
• Intuitive Assay Format: Leverages a straightforward sandwich immunoassay design for high-sensitivity detection.
• Automation-Friendly Design: Engineered for integration with automated liquid handling platforms, streamlining workflows and minimizing manual intervention.
• Specialized Scientific Support: Backed by expert teams with deep experience in SMC^® assay technology.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 940 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 230-240,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 96% Plasma: 130% CSF: 138%
• Recovery: Serum: 102% Plasma: 97% CSF: 91%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although Phospho-Tau (T181) was detectable in mouse, rat, and monkey samples, the assay has not been verified for these species.

Legal Information

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• target_organs: Respiratory Tract
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H373,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation