03-0181-00

SMC^® Human IFN-γ High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IFNɣ in human serum and plasma

• Synonym(e): IFN gamma Single Molecule Counting kit, IFN gamma ultrasensitive immunoassay, interferon gamma immunoassay, interferon immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Eigenschaften

• Produktname: SMC^® Human IFN-γ High Sensitivity Immunoassay Kit,
• description: Used for ultrasensitive quantification of Human IFNɣ in human serum and plasma
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: serum; plasma (K2 EDTA)
• UniProt accession no.: P01579
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Interferon-gamma (IFN-γ) is a key cytokine produced primarily by T cells and natural killer (NK) cells that plays a vital role in the immune response. It is essential for enhancing the immune system′s ability to fight infections, particularly by activating macrophages and promoting the differentiation of T cells into Th1 cells. IFN-γ also has antiviral properties and is involved in the regulation of various immune and inflammatory processes, making it a critical factor in both innate and adaptive immunity.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

• Ultra-Sensitive Detection of IFN-γ: Enables precise quantification of low-level IFN-γ, critical for studying early immune activation, cytokine signaling, and therapeutic response.
• Application in Autoimmune and Inflammatory Disease Research: Supports mechanistic studies of inflammation and immune regulation, including diseases like multiple sclerosis, rheumatoid arthritis, and Castleman disease.
• High Precision and Recovery: Intra-assay CV <15%, inter-assay CV <20%, and recovery rates of 98% in plasma and 104% in serum. Ensures reliable and reproducible data, critical for longitudinal studies, and biomarker validation.
• Specificity for Human IFN-γ: Provides accurate quantification of IFN-γ, supporting mechanistic studies of Th1 responses, macrophage activation, and MHC expression.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 30 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 20-25,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 96% Plasma: 100%
• Recovery: Serum: 104% Plasma: 98%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IFN-γ was detectable in non-human primate, mouse, rat, canine and feline samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B