03-0159-00

SMC^® Human IL-17A High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human IL-17A in human serum and plasma

• Synonym(e): IL-17A Single Molecule Counting Kit, IL-17A immunoassay kit, IL-17A ultra-sensitive immunoassay, Interleukin-17A immunoassay kit
• NACRES: NA.84
• UNSPSC Code: 12161503
• Species reactivity: human
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Eigenschaften

• Produktname: SMC^® Human IL-17A High Sensitivity Immunoassay Kit,
• description: Used for ultrasensitive quantification of Human IL-17A in human serum and plasma
• product line: SMC^®
• usage: kit sufficient for 96 wells
• species reactivity: human
• technique(s): single molecule counting: suitable
• input: plasma (K2 EDTA); serum
• UniProt accession no.: Q16552
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Interleukin-17A (IL-17A) is a pro-inflammatory cytokine primarily produced by Th17 cells, which are a subset of CD4+ T cells. It plays a crucial role in mediating immune responses against extracellular pathogens and is involved in the pathogenesis of various autoimmune and inflammatory diseases, such as rheumatoid arthritis and psoriasis. IL-17A promotes the recruitment of neutrophils and enhances the production of other inflammatory mediators, contributing to the regulation of tissue inflammation and repair.

Application

Inflammatory Disease / Immune Response / Autoimmune Disease

Features and Benefits

• Unmatched Detection Capability: Utilizing SMC^® technology, this assay offers superior performance in quantifying IL-17A in human serum and plasma, ensuring precise and reliable measurements.
• Advance Autoimmune & Inflammatory Disease Research: IL-17A is a key cytokine in Th17-mediated immunity. This assay supports studies in psoriasis, rheumatoid arthritis, IBD, and other chronic inflammatory conditions.
• Enable Early Biomarker Discovery: High sensitivity allows detection of subclinical cytokine shifts, supporting early-stage biomarker identification and therapeutic target validation.
• Improve Data Quality & Confidence: Low background and high signal-to-noise ratio ensure robust, publication-ready data.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 70 fg/mL* (SMCxPRO^® instrument)
• Standard Curve Range: 20-60,000 fg/mL
• Intra-assay CV: <15%
• Inter-assay CV: <20%
• Linearity: Serum: 106% Plasma: 93%
• Recovery: Serum: 92% Plasma: 103%

* Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

Other Notes

Although IL-17A was detectable in rat, mouse, equine, rabbit and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• hcodes: H302 + H332,H311,H319,H360FD,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B