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T2145

Sigma-Aldrich

Tyrode′s Salts

Powder, without sodium bicarbonate, suitable for cell culture

Synonym(e):

Tyrode′s Buffer, Tyrode′s Solution, Tyrode′s buffer

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About This Item

UNSPSC-Code:
12352207
NACRES:
NA.75
Preise und Verfügbarkeit sind derzeit nicht verfügbar.

Produktbezeichnung

Tyrode′s Salts, Without sodium bicarbonate, powder, suitable for cell culture

Form

powder

Methode(n)

cell culture | mammalian: suitable

Lagertemp.

2-8°C

Allgemeine Beschreibung

Tyrode′s Salts play an important role in tissue culture. A salt solution′s basic function is to maintain the pH and osmotic balance in the medium and to provide the cells with water and essential inorganic ions.

Anwendung

Tyrode′s Salts has been used:
  • for seeding cardiomyocytes culture for optical based phenotyping and imaging studies[1]
  • to incubate 3T3-L1 pre-adipocytes in calcium intracellular assays[2]
  • as a medium for the isolation and culturing of myofiber extensor digitorum longus (EDL) muscle[3]

Menge

Formulated to contain 9.6 grams of powder per liter of medium.

Rekonstituierung

Supplement with 1.0 g/L sodium bicarbonate.

Lagerklassenschlüssel

13 - Non Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)


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Die Dokumentenbibliothek aufrufen

Theodora Pavlidou et al.
Stem cells international, 2019, 5980465-5980465 (2019-06-30)
The regeneration of the muscle tissue relies on the capacity of the satellite stem cell (SC) population to exit quiescence, divide asymmetrically, proliferate, and differentiate. In age-related muscle atrophy (sarcopenia) and several dystrophies, regeneration cannot compensate for the loss of
Yu-Fen Chang et al.
PloS one, 12(4), e0174181-e0174181 (2017-04-06)
Identification of drug induced electrical instability of the heart curtails development, and introduction, of potentially proarrhythmic drugs. This problem usually requires complimentary contact based approaches such as patch-clamp electrophysiology combined with field stimulation electrodes to observe and control the cell.
Fernando M Nunez et al.
STAR protocols, 1(3), 100165-100165 (2020-12-31)
Brainstem gliomas are aggressive tumors that are more prevalent in pediatric patients. The location of these tumors makes them inoperable, and currently there is no effective treatment. Recent genomic data revealed the unique biology of these tumors. The following protocol
Anna Marianne Weijler et al.
Frontiers in immunology, 11, 586856-586856 (2020-12-29)
Humoral immunity is a major barrier limiting long-term outcome after organ transplantation. Especially, the production of antibodies directed against donor HLA/MHC antigens (i.e. donor-specific antibodies (DSA)) leading to antibody-mediated rejection (ABMR) is considered to be a major factor negatively affecting
James F X Jones et al.
Experimental physiology, 88(3), 329-334 (2003-04-30)
The cranial venae cavae of the rat heart are composed of cardiac muscle. We tested whether the vagus nerve has an inotropic action on these blood vessels. Stimulation of right or left vagal fibres (n = 7 animals) produced a

Protokolle

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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