SRP0396

DNMT3A/DNMT3L active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥90% (SDS-PAGE)

• NACRES: NA.32
• UNSPSC Code: 12352200
• Assay: ≥90% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf9 cells

Eigenschaften

• biological source: human
• recombinant: expressed in baculovirus infected Sf9 cells
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• mol wt: 34 kDa (MLL1), 52 kDa (DNMT3L)
• packaging: pkg of 10 μg
• NCBI accession no.: NM_013369 (DNMT3L), NM_175629 (DNMT3A)
• UniProt accession no.: Q9Y6K1
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... DNMT3A(1788), DNMT3L(29947)

Beschreibung

General description

The DNMT3A (DNA (cytosine-5)-methyltransferase 3A) gene is mapped to human chromosome 2p23. The encoded protein has 2 isoforms, DNMT3A1 and DNMT3A2. DNMT3A1 is widely expressed and DNMT3A2 is mainly present in embryonic stem cells (ovaries and testes).
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) belongs to the Dnmt3 family. It is unable to methylate DNA. The protein has a cysteine-rich region containing a novel-type zinc finger domain. The gene is mapped to human chromosome 21q22.3.

Application

DNMT3A/DNMT3L active human has been used in in vitro methylation assay and REMSA (RNA electrophoretic mobility shift assay) to study role of ecRNAs (extra coding RNAs) in DNA methylation.[1]

Biochem/physiol Actions

DNMT3A (DNA (cytosine-5)-methyltransferase 3A) is a DNA methyltransferase. It is responsible for the methylation of the cytosine residue in 5′-C-phosphate-G-3′ (CpG) dinucleotides. DNMT3A plays a significant role in immunoregulation (adaptive as well as innate immune responses). It is also the most commonly mutated gene in hematologic malignancies. DNMT3A is a commonly mutated gene in acute myeloid leukemia and is responsible for an unfavorable prognosis.
DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) is mainly responsible for the stimulation of DNA methylation. It is a non-catalytic accessory factor. It helps the DNA methylation machinery to be placed on properly chromatinized DNA regions. It also helps the poorly methylated area, thereby providing uniform methylation patterns.[2] Mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable