S8572

Anti-STIM2 in Kaninchen hergestellte Antikörper

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-stromal interaction molecule 2
• NACRES: NA.41
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 18

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~100 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): western blot: 0.1-0.2 μg/mL using whole extract of mouse brain, western blot: 0.5-1.0 μg/mL using rat brain, western blot: 0.5-1.0 μg/mL using whole extracts of human U87 cells
• UniProt accession no.: Q9P246
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... STIM2(57620) ; mouse ... Stim2(116873) ; rat ... Stim2(117087)

Beschreibung

General description

STIM (stromal interaction molecule) proteins are conserved single-pass transmembrane protein. The three STIM proteins, STIM1, STIM2 and D-Stim, are ubiquitously expressed proteins composed of a single SAM (sterile α-motif) domain and an unpaired EF hand within the highly conserved extracellular region and coiled-coil domains that are conserved in structure and position within the cytoplasmic region. Significant divergence between these proteins was found only at the extreme C-termini. STIM proteins are modified by phosphorylation and N-linked glycosylation.

Immunogen

synthetic peptide corresponding to amino acids 812-833 of human STIM2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Anti-STIM2 antibody produced in rabbit has been used in western blotting.

Biochem/physiol Actions

STIM (stromal interaction molecule) required for the regulation of store-operated Ca²⁺ influx. Store-operated calcium entry is mediated by Ca²⁺ release-activated Ca²⁺ (CRAC) channels, following Ca²⁺ depletion from the endoplasmic reticulum (ER) stores. This process is crucial for gene transcription, proliferation and cytokine release. STIM2 is an inhibitor of STIM1-mediated store-operated Ca²⁺ entry. STIM2 co-localizes with STIM1 in punctae upon store depletion, and coprecipitates with STIM1 from cell lysates, suggesting that they may play a coordinated role in controlling Ca²⁺ entry.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)