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Merck

S2519

Sigma-Aldrich

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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About This Item

MDL-Nummer:
UNSPSC-Code:
12352203
NACRES:
NA.46

Biologische Quelle

rabbit

Qualitätsniveau

Konjugat

unconjugated

Antikörperform

fractionated antiserum

Antikörper-Produkttyp

primary antibodies

Klon

polyclonal

Form

buffered aqueous solution

Speziesreaktivität

soy

Methode(n)

dot blot: 1:500
indirect ELISA: 1:5,000

Versandbedingung

dry ice

Lagertemp.

−20°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.
Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues.

Immunogen

renatured soy protein extracted from soy acetone powder with hot urea.

Anwendung

Anti-Soy Protein antibody produced in rabbit has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • supershift analysis
  • fluorescent imaging
  • immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Biochem./physiol. Wirkung

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

Zielbeschreibung

Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.

Physikalische Form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Lagerklassenschlüssel

10 - Combustible liquids

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable


Analysenzertifikate (COA)

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Activation of the signal transducers and activators of transcription signaling pathway by growth hormone (GH) in skin fibroblasts from normal and GH binding protein-positive Laron Syndrome children
Freeth JS, et al.
Endocrinology, 139(1), 20-28 (1998)
Flight movement and spatial distribution of immunomarked thrips in onion, potato, and tomato
Fernandes FL and Fernandes MES
Pesquisa Agropecuaria Brasileira, 50(5), 399-406 (2015)
J S Freeth et al.
Endocrinology, 139(1), 20-28 (1998-01-08)
We have previously described two families (H and M) with GH binding protein-positive Laron Syndrome (LS), proposed to have one or more post GHR signaling defects. In the present study, we have examined whether the signal transducers and activators of
Priyae Brath Gautam et al.
Journal of food science and technology, 54(13), 4213-4219 (2017-12-01)
A lateral flow based detection method for ascertaining the presence of soymilk in whole bovine milk has been described. The method uses commercially available rabbit anti-soy protein antibodies conjugated to gold nanoparticles (AuNPs) wherein soymilk protein in adulterated milk and
S M Potter et al.
The American journal of clinical nutrition, 68(6 Suppl), 1375S-1379S (1998-12-16)
The effects of soy protein (40 g/d) containing moderate and higher concentrations of isoflavones on blood lipid profiles, mononuclear cell LDL receptor messenger RNA, and bone mineral density and content were investigated in 66 free-living, hypercholesterolemic, postmenopausal women during a

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