This product is a heterogeneous mixture of tRNA molecules This material has not been sequenced.
Fortfahren mit
R6625
Ribonukleinsäure aus Torulahefe
Type VI
Synonym(e):
RNA
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 25 g | Warenkorb auf Verfügbarkeit prüfen | € 194,00 | |
| 100 g | Warenkorb auf Verfügbarkeit prüfen | € 586,00 | |
| 1 kg | Warenkorb auf Verfügbarkeit prüfen | € 2.850,00 |
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Storage temp.:
−20°C
Technischer Dienst
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Unterstützung erhaltenGeneral description
Ribonukleinsäure (RNA) aus Torula-Hefe ist das Substrat für das RNase-Enzym. Die Isolierung von RNA aus Hefe ist ein kompliziertes Verfahren und involviert Zyklen von Erwärmen und Einfrieren von Zellen in Gegenwart von Phenyl und Detergenzien (SDS).[1]
Application
Ribonukleinsäure (RNA) eignet sich für Anwendungen wie Northern-Blot-Hybridisierung, Reverse-Transkriptase-Polymerase-Kettenreaktion (RT-PCR) und cDNA-Konstruktion.[1]
Ribonukleinsäure (RNA) aus Torula-Hefe kann als Substrat zur Untersuchung der Ribonukleaseaktivität von Enzymen wie Ribonuklease-A, Ribonuklease T1 (RNase) und des antiviralen Proteins 1 der Bougainvillea x buttiana (BBAP1) verwendet werden.
Ribonukleinsäure (RNA) aus Torula-Hefe kann als Substrat zur Untersuchung der Ribonukleaseaktivität von Enzymen wie Ribonuklease-A, Ribonuklease T1 (RNase) und des antiviralen Proteins 1 der Bougainvillea x buttiana (BBAP1) verwendet werden.
Analysis Note
Phosphor: 8,0–10,0 % (wasserfrei)
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Stephanie L Grillo et al.
Purinergic signalling, 15(3), 327-342 (2019-07-06)
Adenosine is an endogenous nucleoside in the central nervous system that acts on adenosine receptors. These are G protein-coupled receptors that have four known subtypes: A1, A2A, A2B, and A3 receptors. In the present study, we aimed to map the
Michael R Green et al.
Cold Spring Harbor protocols, 2021(12) (2021-12-03)
Isolation of RNA from yeast is complicated by the need to first break the thick, rigid cell wall. The protocol provided here uses a cycle of heating and freezing of cells in the presence of phenol and the detergent sodium
Jay S Petrick et al.
Regulatory toxicology and pharmacology : RTP, 81, 57-68 (2016-10-30)
Genetically modified (GM) crops have been developed and commercialized that utilize double stranded RNAs (dsRNA) to suppress a target gene(s), producing virus resistance, nutritional and quality traits. MON 87411 is a GM maize variety that leverages dsRNAs to selectively control
Global Trade Item Number
| SKU | GTIN |
|---|---|
| R6625-1KG | 04061836695910 |
| R6625-100G | 04061836695903 |
| R6625-25G | 04061836695927 |
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What are the nucleotide sequences of the mixture of RNA?
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How many base pairs are in the RNA? Can I dossolve it in TE buffer?
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This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 daltons which corresponds to approximately 10 to 120 base pairs in size. The material is tested for solubility at 10 mg per 1 ml of 0.1M Acetate buffer, pH 5.0. Testing is TE buffer is not performed. However, this buffer is commonly used as short term storage buffer for RNA.
Please see the link below to review the oligo handling and stability guide for additional information: https://www.sigmaaldrich.com/US/en/technical-documents/protocol/genomics/dna-and-rna-purification/oligonucleotide-handling-and-stability
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I would like to know if this product RNA from torula yeast (R6625) contains only mRNA and rRNA or does it also contains transfer-RNA?
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This product is not assayed for the distribution of RNA forms, but it is expected to be a heterogeneous mixture ranging from 3000 to 40000 Daltons.
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What does type VI mean?
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The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.
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What does Type IV refer to?
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The 'type' designation for this product is an internal definition of Sigma and has no specific biological significance. It is in reference to the method of manufacture or product preparation.
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What is the molecular weight of this product? What is the RNA concentration?
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This product is a heterogeneous mixture of RNA molecules ranging from 3000 to 40000 in MW. The material is not tested for purity. There is a maximum allowable water content specification of 10%. Please see the link below for review a sample Certificate of Analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/R6625-BULKWXBF1071V.pdfHelpful?
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What species is Product R6625, Ribonucleic acid from torula yeast, from?
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The former taxonomic name for Torula yeast is Torulopsis utilus; the current name is Candida utilus.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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