R1656

RAF1 (EE) (306-end), active, GST tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): CMD1NN, NS5, RAF proto-oncogene serine/threonine-protein kinase, cRaf
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 5100-6900 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Biological source: fermentation/recombinant
• Recombinant: expressed in baculovirus infected Sf9 cells

Eigenschaften

• biological source: fermentation/recombinant
• Quality Segment: 200
• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 5100-6900 nmol/min·mg
• mol wt: ~63 kDa
• manufacturer/tradename: Precision
• technique(s): cell based assay: suitable
• solubility: water: soluble
• Protein ID accession no.: NM 002880
• UniProt accession no.: P04049
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... RAF1(5894)

Beschreibung

General description

Research area: Cell Signaling

Rapidly Accelerated Fibrosarcoma (RAF) belongs to receptor tyrosine kinase (RTK) family. RAF exists as three isoforms A, B and C-RAF or RAF-1. RAF1 links Ras family members with mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) signaling cascade.[1] The RAF-1 gene is mapped to the human chromosome at 3p25.2.[2]

Biochem/physiol Actions

Rapidly Accelerated Fibrosarcoma 1(RAF1), a member of MAP kinase kinase kinase (MAP3K) family plays a vital role in cell signaling by directly interacting with GTPases and functions downstream in their signaling pathway. RaF1 is activated by Ras in the presence of growth-promoting signals, leading to the activation of mitogen-activated protein kinase kinase (MEK1/MEK2) and extracellular signal-regulated protein kinases 1 and 2 (ERK1/ERK2) downstream. The activated ERK proteins control gene expression related to cell division, apoptosis, cell differentiation, and cell migration. The gene is mutated or upregulated in prostate and bladder cancer. Also, mutations are associated with Noonan syndrome, which leads to various physical abnormalities like craniofacial dysmorphisms, ocular, and musculoskeletal irregularities, neurocognitive impairment, and cardiac malformations.

Physical form

Supplied in 50 mM Tris-HCl, pH 7.5, with 150 mM NaCl, 0.2 5mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, and 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)