P6056

Endoproteinase Arg-C aus Maussubmaxillardrüse

suitable for protein sequencing, lyophilized powder

• CAS-Nummer: 82047-85-6
• UNSPSC Code: 12352202
• NACRES: NA.56
• EC Number: 279-893-6
• MDL number: MFCD01324999
• EG-Nummer: 3.4.21.35 (BRENDA, IUBMB)

Eigenschaften

• form: lyophilized powder
• Quality Level: 200
• packaging: vial of 5 μg
• suitability: suitable for protein sequencing
• storage temp.: −20°C

Beschreibung

Application

Endoproteinase Arg-C from mouse submaxillary gland has been used to study the syncytium formation in MERS-CoV (middle East respiratory syndrome coronavirus)-infected Vero cells in the presence of exogenous proteases. It has been used for the digestion of Rpl23ab (ribosomal protein L23ab)-containing fraction for LC-MS (liquid chromatography–mass spectrometry)/MS analysis.

Biochem/physiol Actions

Endoproteinase Arg-C is a serine endoprotease from mouse submaxillary gland which hydrolyzes peptide bonds at the carboxyl side of arginyl residues. The enzyme has been shown to cleave Lys-Lys and Lys-Arg bonds, and all Arg-X bonds may not be hydrolyzed.

Other Notes

Eine Einheit hydrolysiert 1.0 μmol Nα-p-Tosyl-L-arginin-methylester pro Min. bei pH 8.0 und 25°C.

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3