Fortfahren mit
P4300
Pektinase aus Rhizopus sp.
suitable for plant cell culture, BioReagent, crude powder
Synonym(e):
Poly-(1,4-α-D-galacturonide) glycanohydrolase, Polygalacturonase
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Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352200
EC Number:
232-885-6
MDL number:
product line
BioReagent
form
crude powder
specific activity
400-800 units/g solid
technique(s)
cell culture | plant: suitable
storage temp.
−20°C
General description
Pectinase P4300 is a natural pectinase (polygalacturonase) derived from fungus Rhizops sp. that randomly hydrolyzes the (1→4)-α-D-galactosiduronic linkages in pectate and other galacturonans. This enzyme breaks down pectin found in the middle lamella of plant cell walls. Pectinase helps facilitate the maceration of plant materials and extraction processes. Pectinase P4300 is used in plant protoplast preparation to digest cell wall prior to organelle isolation.
Application
Zur Herstellung von Pflanzenprotoplast für den Zellwandabbau vor der organellen Isolation.
Other Notes
One unit will liberate 1.0 μmole of galacturonic acid from polygalacturonic acid per min at pH 4.0 at 25 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Jeffrey A Mertens et al.
Fungal genetics and biology : FG & B, 45(12), 1616-1624 (2008-10-22)
A search of the recently sequenced Rhizopus oryzae strain 99-880 genome database uncovered 18 putative polygalacturonase genes with two genes being identical and only one with similarity to a previously reported R. oryzae polygalacturonase gene. The 17 different genes share
Shigenobu Yoshida et al.
Mycological research, 108(Pt 12), 1407-1414 (2005-03-11)
The polygalacturonase (PG)-encoding gene (rpg1) of Rhizopus oryzae, the causal pathogen of rhizopus rot of mulberry, was cloned and sequenced. PGs were partially purified from incubation mixture of 2% pectin medium and their N-terminal amino acid sequences were determined by
K Saito et al.
Journal of industrial microbiology & biotechnology, 30(7), 440-444 (2003-07-05)
Rhizopus oryzae strain NBRC 4707 produced lactic acid and ethanol more efficiently than strain NRRL 395 in potato pulp, an agricultural by-product of the starch industry. The two strains developed comparable activities of xylanase, cellulase, alpha-amylase, and glucoamylase, while the