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MAK025

Eisen-Assay-Kit

sufficient for 100 colorimetric tests

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Über diesen Artikel

NACRES:
NA.84
UNSPSC Code:
12161503

usage

sufficient for 100 colorimetric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric

relevant disease(s)

cancer; hematological disorder

storage temp.

−20°C

General description

Eisen ist ein Mineral, das eine wesentliche Rolle in vielen biologischen Prozessen spielt, zum Beispiel bei der DNA-Synthese, beim Elektronentransport und beim Sauerstofftransport. Eisen ist ein Übergangselement, das eine Reihe von Oxidationsstufen bilden kann, wobei die häufigsten Eisen II (Fe2+ bzw. zweiwertiges Eisen) und Eisen III (Fe3+ bzw. dreiwertiges Eisen) sind. Eisenhaltige Proteine sind an vielen Reaktionen beteiligt, wobei oft vorübergehende Änderungen des Oxidationszustandes von Eisen zur Durchführung chemischer Reaktionen genutzt werden.

Application

Für die Messung der Eisenkonzentration in verschiedenen Proben geeignet.
Zur Bestimmung der Konzentration von Fe2+ in Wildtyp- (WT-) und Δ Eisenaufnahmeregulator-Stämmen (Fur-Stämmen) von A. baumannii wurde ein Eisen-Assay-Kit verwendet.

Biochem/physiol Actions

Bei diesem Assay wird Eisen durch die Zugabe eines sauren Puffers freigesetzt. Proben können zur Messung von Fe2+ direkt getestet werden oder zur Messung des Gesamteisens (Fe2+ und Fe3+) reduziert werden. Freigesetztes Eisen wird mit einem Chromagen umgesetzt, wodurch ein kolorimetrisches Produkt (593 nm) entsteht, das proportional zum vorhandenen Eisen ist. Das Eisen-Assay-Kit bietet ein einfaches und praktisches Verfahren zur Messung von Eisen in verschiedenen biologischen Proben.

Preparation Note

Das Kit ist mit Serum- und Gewebeproben, jedoch nicht mit Plasmaproben kompatibel.

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pictograms

Corrosion
GHS05

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

wgk

WGK 2

Lagerklasse

10 - Combustible liquids

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
8H01K03900
8A26K03900
8C21K03900
8K12K03900
7H27K03900

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Verwandter Inhalt

Manman Xu et al.
Journal of cellular physiology, 234(4), 4232-4242 (2018-08-23)
Previous studies have demonstrated an effect of estrogen on iron metabolism in peripheral tissues. The role of estrogen on brain iron metabolism is currently unknown. In this study, we investigated the effect and mechanism of estrogen on iron transport proteins.
Contributions of ferric uptake regulator Fur to the sensitivity and oxidative response of Acinetobacter baumannii to antibiotics
Ajiboye T o, et al.
Microbial Pathogenesis, 119, 35-41 (2018)
Ems T, Huecker MR
Biochemistry, Iron Absorption (2018)
Alle zugehörigen Dokumente anzeigen

Global Trade Item Number

SKUGTIN
MAK025-1KT04061834064800

Questions

1–7 of 7 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. Is the MAK025 Iron Assay Kit able to detect iron when it is present as ferric ammonium citrate in Mycobacterium tuberculosis 7H9 broth?

    1 answer

    1. In the assay, ferric carrier protein will release ferric ions into the solution when introduced to an acidic buffer. Once these ions are reduced to the ferrous form (Fe2+), they react with Ferene S, forming a stable colored complex detectable at an absorbance of 593 nm. Consequently, the kit should theoretically be capable of detecting iron in the form of ferric ammonium citrate. It is designed to measure iron within a linear range of 0.4 to 10 nmol, or 8 µM to 200 µM iron concentration in various samples.
      It should be noted that the concentration of ferric ammonium citrate in Middlebrook 7H9 broth is generally 0.04 g/L, equivalent to 150 µmol/liter.

      Helpful?

  4. Can this assay quantify iron contained within heme directly, or is it necessary to first release the iron from the heme for detection?

    1 answer

    1. Iron attached to carrier proteins like transferrin can be quantified with the kit because these proteins will release ferric ions into the solution within an acidic buffer. However, proteins that contain iron, such as those in heme, do not dissociate in this way. As a result, it is not anticipated that iron bound within heme can be measured using this particular assay kit.

      Helpful?

  5. How can the detection of iron in mouse brain homogenate be improved when using the assay kit, given that current measurements are near or below the detection limit?

    1 answer

    1. It is suggested to reduce the quantity of homogenization buffer. For effective homogenization and cell lysis, employing a dounce homogenizer or applying mild sonication is recommended. Furthermore, using a greater quantity of tissue with a reduced amount of buffer might prove to be beneficial.

      Helpful?

  6. To homogenize mouse cells to evaluate iron content with "Iron assay kit", which type of homogenization method is best suited? Thanks

    1 answer

    1. It is recommended to use a Dounce homogenizer, not a "French press" or sonicator, or detergents or beads, for homogenization.

      Helpful?

  7. What is the range of iron concentration that can be measured?

    1 answer

    1. The detection range of this kit is 8 µM - 200 µM.

      Helpful?

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