M9445

Matrix Metalloproteinase-2 aus mouse

recombinant, expressed in NSO cells, >95% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): 72 kD Gelatinase, 72 kD Type IV Collagenase, Gelatinase A, MMP-2
• UNSPSC Code: 12352202
• NACRES: NA.32
• MDL number: MFCD01324322

Eigenschaften

• recombinant: expressed in NSO cells
• Quality Segment: 200
• assay: >95% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• mol wt: apparent mol wt ~72 kDa
• UniProt accession no.: P33434, P33436
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Mmp2(17390) ; rat ... Mmp2(81686)

Beschreibung

General description

Matrix Metalloproteinase-2 (MMP-2) also known as gelatinase or type IV collagenase is a 72kDa protein. MMP-2 is a member of matrix metalloproteinase (MMP) family of enzymes.[1] Basic structure of MMP2 contains signal peptide domain that targets the enzyme for secretion, the pro-peptide domain, which is removed when the enzyme is activated and the catalytic site containing gelatin-binding domain.

Application

Matrix metalloproteinase-2 (MMP2) human has been used as a standard in zymography to measure proteolytic activity of MMP-2.

Biochem/physiol Actions

MMP-2 degrades general matrix components and may have a role in processes such as host defense, cell proliferation, and protein turnover as well as tissue remodeling.

The amino acid sequences 1-662[2] of the proenzymes of MMP-2 are identical between mouse and rat.

Matrix Metalloproteinase-2 (MMP-2) cleaves gelatin, type IV, V, VII, X, and XI collagens, fibronectin, elastin, laminin, proteoglycans and a range of non extracellular matrix (ECM ) components. MMP-2 cleaves native type I collagen to N-terminal ¾ and C-terminal ¼ fragments identical to those generated by interstitial collagenases.[1] MMP2 and MMP9 play an essential role in matrix degradation and they are implicated in the maintenance of neovascularization. In mice, deletion or inhibition of MMP2 protects against myocardial rupture.

Physical form

Supplied as a 0.2 μm filtered solution of 25 mM Tris, pH 7.5, 5 mM calcium chloride, 75 mM sodium chloride, 0.025% Brij^® 35 and 50% glycerol.

Analysis Note

The biological activity is measured by its ability to cleave a fluorogenic peptide sustrate.

Legal Information

Brij is a registered trademark of Croda International PLC

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)