This product is methylated Jurkat DNA (T cell leukemia). Unfortunately, we do not evaluate the molecular weight of the product. However, it's genomic DNA.
Fortfahren mit
M8570
Methylated Control DNA
50 ng/μL
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Über diesen Artikel
NACRES:
NA.55
UNSPSC Code:
23201100
Form:
liquid
Storage temp.:
−20°C
form
liquid
concentration
50 ng/μL
color
colorless
storage temp.
−20°C
General description
Fully methylated Jurkat DNA. 5-methyl-cytidine (5mC) content determined by mass spectrometry. See lot specific certificate of analysis or vial label for 5mC content of each lot.
Application
Methylated Control DNA has been used as a negative control in bisulfite sequencing of embryonic genomic DNA.
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| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
Lagerklasse
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Hello. Is this product a whole genomic DNA? Is it fragmented?
1 answer-
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What buffer is the Methylated Control DNA dissolved in?
1 answer-
This product is dissolved in a TE buffer consisting of 10 mM Tris-HCl with 1 mM EDTA.
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It has been written 50 ng/ul for concentration. What is the total volume (as ul or ml) and total probe amount (as ug or mg) in the vial. Could you inform me, please
1 answer-
The total volume is 16ul.
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What is the percentage of 5-Methylcytosine?
1 answer-
As mentioned on the product detail page, the % 5mC content is reported in the lot specific Certificate of Analysis, as well as the product label. The specification for 5mC is >/= 4.5%. The historical average is approximately 6.3%. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/M8570-1VLSLCK7031.pdf
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