M5814

MKK6/SKK3, active human

≥80% (SDS-PAGE), recombinant, expressed in E. coli, Maltose binding protein tagged, buffered aqueous solution

• Synonym(e): MAP Kinase Kinase 6, MAP Kinase Kinase 6/SKK 3
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD03457508
• Assay: ≥80% (SDS-PAGE)
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Level: 200
• assay: ≥80% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: 81 kDa
• UniProt accession no.: P52564
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... MAP2K6(5608)

Beschreibung

Application

Active enzyme for study of stress-activated protein kinases.

May be used for activation of p38α followed by phosphorylation of MBP.

Biochem/physiol Actions

Active in the phosphorylation of p38/SAPK2a

Physical form

Solution in approx. 1 mg/ml in 50 mM Tris-HCl, pH 7.5, 0.1 mM EGTA, 0.1% 2-mercaptoethanol, 0.15 M NaCl, 0.03% Brij^® L23, 270 mM sucrose, 0.2 mM PMSF, 1 mM benzamidine.

Other Notes

1 unit will activate 1 unit of p38/SAPK2 per minute. 1 unit of active p38/SAPK2 will incorporate 1 nanomole of phosphorus into myelin basic protein per minute.

Legal Information

Brij is a registered trademark of Croda International PLC

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable