L4385

α-Lactalbumin aus Kuhmilch

Marker for non-denaturing PAGE

• CAS-Nummer: 9051-29-0
• UNSPSC Code: 12352202
• EC Number: 235-747-3
• NACRES: NA.61
• MDL number: MFCD00162025
• Biological source: bovine milk
• Form: lyophilized powder
• Technique(s): microbiological culture: suitable
• Concentration: 0.9—1.4 mg per vial protein (biuret)

Eigenschaften

• biological source: bovine milk
• Quality Segment: 200
• form: lyophilized powder
• mol wt: ~14.2 kDa
• concentration: 0.9—1.4 mg per vial protein (biuret)
• technique(s): microbiological culture: suitable
• UniProt accession no.: P00711
• storage temp.: −20°C
• Gene Information: cow ... LALBA(281894)

Beschreibung

General description

α-Lactalbumin is a small, globular, whey protein that has been found in all milk studied to date. It is a metalloprotein of approximately 14 kDa produced in the mammary glands.[1]

Application

α-Lactalbumin was used in the isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species.[1] It was also used in a study to test if the neuroendocrine protein 7B2 suppresses the aggregation of neurodegenerative disease-related proteins.[2]

Biochem/physiol Actions

Verändert die Substratspezifität von Galactosyltransferase und beschleunigt so die Lactosebildung; der Komplex aus Galactosyltransferase und α-Lactalbumin wird Lactose-Synthase genannt.

Verändert die Substratspezifität von Galactosyltransferase und beschleunigt so die Lactosebildung; der Komplex aus Galactosyltransferase und α-Lactalbumin wird Lactose-Synthase genannt. Ortsgerichtete Mutagenese von Asp⁸⁷ oder Asp⁸⁸ an Ala hebt die starke Calcium-Bindungsaffinität vollständig auf und reduziert die Stimulation von Lactose-Synthase auf <3,5 % der Maximalrate.

α-Lactalbumin is the cheif protein in human milk. It consists of a single polypeptide chain with 8 cysteines which form disulfide bridges. α-Lactalbumin binds several metal ions, including calcium, which is thought to play a role in the regeneration of native α-lactalbumin from the reduced, denatured form.[3] α-Lactalbumin also has a distinct zinc binding site that is thought to play a role in the binding of the lactose synthase complex. [4] [5]The mature protein consists of 123 amino acid residues (14 kD), and it has a three-dimensional structure with 1.7 Α° resolution, demonstrating four α-helices and a triple stranded antiparallel β-sheet.[6]

Preparation Note

0.9-1.4 mg/mL nach der Rekonstitution mit 1 mL Wasser

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable