L4021

Prolaktin human

recombinant, expressed in E. coli, lyophilized powder, BioReagent, suitable for cell culture, >97% (SDS-PAGE)

• Synonym(e): hPRL, LTH, Lactogenic Hormone, Luteotropic Hormone, PRL
• CAS-Nummer: 9002-62-4
• UNSPSC Code: 51111800
• NACRES: NA.77
• MDL number: MFCD00131553
• Biological source: Escherichia coli
• Form: lyophilized powder
• Assay: >97% (SDS-PAGE)

Eigenschaften

• biological source: Escherichia coli
• Quality Segment: 200
• recombinant: expressed in E. coli
• description: 200 amino acid residue methionyl form of mature, recombinant human prolactin (amino acid residues 29-227).
• product line: BioReagent
• assay: >97% (SDS-PAGE)
• form: lyophilized powder
• potency: 0.030-0.300 ng/mL EC₅₀
• mol wt: predicted mol wt ~24 kDa
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• solubility: water: 0.5000 mL, clear, colorless
• UniProt accession no.: P01236
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... PRL(5617)

Beschreibung

Application

Use in cell culture applications to study prolactin regulation of cell signaling and gene expression.

Biochem/physiol Actions

Neuroendocrine hormone; belongs to the cytokine hematopoietic receptor family; induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

Prolactin is a neuroendocrine hormone. The prolactin receptor is a transmembrane glycoprotein that belongs to the cytokine hematopoietic receptor family. A large number of cells and organs express the receptor, including B cells, T cells, macrophages, monocytes, and neutrophils. Prolactin signal transduction involves the JAK/STAT families and the src kinase family. Induces lactation; inhibits secretion of gonadotropins; release is inhibited by dopamine.

Physical form

Lyophilized from a solution in 10 mM phosphate and 50 mM sodium chloride, pH 8.0.

Preparation Note

It is recommended that sterile 4 mM HCl containing 1 mg/mL bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.

Analysis Note

The biological activity is assayed by proliferation of the rat lymphoma cell line, Nb2-11.

Other Notes

Contains an N-terminal methionine not present in the natural product.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H360
• pcodes: P201 - P308 + P313
• Hazard Classifications: Repr. 1B
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable