Viral testing is not performed on this product; only bacteriological growth is tested.
Fortfahren mit
L2542
LB-Bouillon (Miller)
Liquid microbial growth medium
LB Broth (Miller)
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(e):
Luria-Bertani-Bouillon, Luria-Bouillon, Miller, Miller
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
500 mL | Warenkorb auf Verfügbarkeit prüfen | € 47,40 |
Über diesen Artikel
NACRES:
NA.85
UNSPSC Code:
41106200
Technischer Dienst
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Unterstützung erhaltengrade
Molecular Biology
Quality Segment
sterility
sterile; 0.2 μm filtered
form
liquid
technique(s)
microbiological culture: suitable
pH
6.5-7.2
application(s)
microbiology
storage temp.
room temp
suitability
nonselective for Escherichia coli, nonselective for coliforms
General description
Die LB-Bouillon nach Miller ist ein gerne genutztes Nährmedium für das Mikrobenwachstum, das auch für die Kultivierung von E. coli verwendet wird. Die nährstoffreiche mikrobielle Bouillon enthält Peptide, Aminosäuren, wasserlösliche Vitamine und Kohlenhydrate. Sie ermöglicht schnelles Wachstum, verbessert die Wachstumsausbeuten bei verschiedenen Bakterienarten und kann das hydrophile Verhalten von Proben über längere Zeiträume aufrechterhalten.
Application
LB-Bouillon (Miller) eignet sich für:
Features and Benefits
Flüssige LB nach Miller bietet folgendes:
- Gefiltertes und sterilisiertes gebrauchsfertiges Format
- Praktische Packungsgröße
- Standardmäßige Formulierung
Other Notes
10 g/l Pepton
10 g/l NaCl
5 g/l Hefeextrakt
10 g/l NaCl
5 g/l Hefeextrakt
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| technique(s) microbiological culture: suitable | technique(s) - | technique(s) microbiological culture: suitable | technique(s) microbiological culture: suitable |
| form liquid | form powder | form powder | form powder |
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility non-sterile | sterility non-sterile |
| application(s) microbiology | application(s) food and beverages | application(s) food and beverages | application(s) food and beverages |
| grade Molecular Biology | grade Molecular Biology | grade Molecular Biology | grade Molecular Biology |
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Lagerklasse
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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is any viral testing performed on the LB medium
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Is this product defined as animal free component?
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This product is not defined as an animal-free component.
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Which bacterial culture medium is the best choice for my application?
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Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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Will adding magnesium to the culture medium increase cell density?
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In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
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What is the difference among the LB - Miller products?
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LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What are the differences among the Luria, Lennox and Miller LB formulations?
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LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
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