I5885

Monoklonaler Anti-Human IgG(γ-kettenspezifisch)-Antikörper

clone GG-5, ascites fluid

• Synonym(e): Monoclonal Anti-Human IgG (γ-chain specific)
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: GG-5, monoclonal
• Application: ELISA (i)
• Citations: 8

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: GG-5, monoclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:20,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Bindet Human-IgG, bindet keine anderen Human-Ig. Der Antikörper ist spezifisch für eine Determinante an der Schwerkette von Human-IgG. Der Antikörper bildet in der Flüssigphase in Gegenwart von 3 % PEG 6000 Antigen-Antikörper-Komplexe.

Monoclonal Anti-Human IgG (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Immunoglobulins have two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is usually found as a monomer. IgG is further subdivided into four classes namely, IgG1, IgG2, IgG3, and IgG4. About 70 percent of the total immunoglobulin consists of IgG.

Application

ELISA-Assays wurden mit alkalischer Phosphatase, die an monoklonales Anti-Human-IgG der Maus konjugiert ist, durchgeführt, um Antikörper gegen verschiedene S. Aureus-Antigene im Serum von gesunden Individuen zu finden. Der Antikörper wurde in PBSt auf 1:30.000 verdünnt und mit Proben 2 Std. bei 37 °C inkubiert. Reaktin wurde mit p-Nitrophenylphosphatsubstrat (Sigma) entwickelt.

Flüssigpräzipitat (Lppt).

Monoclonal Anti-Human IgG (γ-chain specific) antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA).[1]

Biochem/physiol Actions

Immunoglobulin G (IgG) mainly helps in immune defense. IgG participates in hypersensitivity type II and type III.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable