G3046

GDF-15 human

• Synonym(e): Growth Differentiation Factor 15 human, MIC-1, Macrophage inhibitory cyotkine-1
• CAS-Nummer: 1821355-80-9
• NACRES: NA.26
• UNSPSC Code: 12352202
• Form: lyophilized powder
• Assay: >97% (visualized with silver stain, SDS-PAGE)
• Biological source: human
• Recombinant: expressed in CHO cells
• Mol wt: dimer 13 kDa (based on N-terminal sequencing, starting at the first histidine of the 6X histidine tag)

Eigenschaften

• biological source: human
• Quality Segment: 100
• recombinant: expressed in CHO cells
• assay: >97% (visualized with silver stain, SDS-PAGE)
• form: lyophilized powder
• mol wt: dimer 13 kDa (based on N-terminal sequencing, starting at the first histidine of the 6X histidine tag)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• UniProt accession no.: Q99988
• storage temp.: −20°C
• Gene Information: human ... GDF15(9518)

Beschreibung

General description

Growth-differentiation factor-15 (GDF-15) is a stress-responsive member of the transforming growth factor-β cytokine superfamily. It is also known as PTGF-Growth-differentiation factor-15 (GDF-15) and is a stress-responsive member of the transforming growth factor-β cytokine superfamily.² It is also known as PTGF-β, TGF-PL or MIC-1β, TGF-PL or MIC-1. [1]

Application

Growth-differentiation factor-15 (GDF-15) has been associated in the regulation of cell survival, proliferation, and differentiation and also in the protection from ischemia/reperfusion injury. Inflammatory and apoptotic pathways are regulated in injured tissues by GDFs during disease processes.[2]

Biochem/physiol Actions

Although it has been classified as a divergent member of the transforming growth factor-β superfamily, GDF-15 shares structural characteristics with this superfamily. It has been identified as a biomarker for human prostate cancer. Additionally, increased GDF-15 expression has been observed in breast, pancreas and colorectal cancers. Functions of this cytokine include inhibition of TNF-α production, initiation of cartilage formation, and promotion of neuronal survival.
It has been discussed to serve as a secreted biomarker for activation of the p53 pathway in human cancer.[1]

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile, 0.1% trifluoroacetic acid containing 50 μg of bovine serum albumin per 1 μg of GDF-15.

Analysis Note

Under reducing conditions in SDS-PAGE, each recombinant GDF-15 monomer migrates as an ~14 kDa protein.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable