F9554

Anti-Fatty Acid Synthase antibody produced in rabbit

1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-FAS, Anti-FASN
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02262888
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 14

Eigenschaften

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 270 kDa
• species reactivity: mouse, rat, human (predicted)
• concentration: 1 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using lysates of mouse myoblast C2 cells, western blot: 0.5-1 μg/mL using lysates of rat hepatocytoma FAO cells
• UniProt accession no.: P49327
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FASN(2194) ; mouse ... Fasn(14104) ; rat ... Fasn(50671)

Beschreibung

General description

Fatty Acid Synthase encodes an enzyme that is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids.

Fatty acid synthase (FAS, FASN) is encoded by the gene mapped to human chromosome 17q25.3.[1] In mammals, it is composed of two identical multifunctional polypeptides, in which three catalytic domains in the N-terminal section (β-ketoacyl synthase, malonyl/acetyl tansferase, and dehydrase) are separated by a core region of about 600 residues from four C-terminal domains (enoyl reductase, β-ketoacyl reductase, acyl carrier protein, and thioesterase).

Immunogen

synthetic peptide corresponding to amino acids 1982-1999 of mouse fatty acid synthase, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide is conserved in rat and differs from the human sequence by three amino acids.

Application

Anti-Fatty Acid Synthase antibody is suitable for western blot analysis at a concentration of 0.5-1μg/mL using lysates of mouse myoblast C2 cells and rat hepatocytoma FAO cells.

Fatty acid synthase antibody produced in rabbit has been used in immunohistochemistry and western blot analysis.

Biochem/physiol Actions

FAS plays an essential role during embryogenesis and energy homeostasis in adult animals. It also acts as the carbon source for fatty acid synthesis. It has been shown to be overexpressed in various types of human cancer, including prostate, breast, ovary and others. FASN is a multifunctional protein, mainly involved in catalyzing the synthesis of palmitate from acetyl-CoA and malonyl CoA, in the presence of NADPH.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)