F7680

FAK (393-698), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(e): FADK, FAK1, PTK2, pp125FAK
• NACRES: NA.32
• UNSPSC Code: 12352204
• Specific activity: 221-299 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells

Eigenschaften

• recombinant: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 221-299 nmol/min·mg
• mol wt: ~35 kDa
• UniProt accession no.: Q05397
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PTK2(5747)

Beschreibung

General description

Focal adhesion kinase (FAK) or PTK2 (protein tyrosine kinase 2) is a tyrosine kinase, which resides in the cytoplasm, and concentrated at cellular focal points. It is a highly conserved 125kDa protein, which has a wide range of tissue expression. It is composed of 1,052 amino acids, and contains N- and C-termini flanking the central kinase domain. The N-terminal called the FERM (four-point-one, ezrin, radixin, moesin) domain, controls FAK activity, whereas the C-terminal contains the focal-adhesion-targeting (FAT) domain.[1]

Biochem/physiol Actions

Focal adhesion kinase (FAK) or PTK2 protein tyrosine kinase 2 plays multiple roles such as, control of transcription, cell cycle progression, apoptosis, cell migration, and metastasis in case of tumor cells. This attributes to its predominant role in cell signaling. It is involved in the infection of rabies virus (RABV).[1] Studies in Spanish population show that polymorphisms rs7460 and rs7843014 in this gene might be linked with exceptional longevity.[2] Phosphatidylinositol 4-phosphate 5-kinase type Iγ enzyme produces phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2] in focal adhesion, which cross-links FAK activation to integrin signaling.[3]

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)