F4890

Monoclonal Anti-Rabbit Immunoglobulins–FITC antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: FITC conjugate
• Clone: RG-16, monoclonal
• Application: IF, IHC (p)
• Citations: 17

Eigenschaften

• biological source: mouse
• Quality Level: 200
• conjugate: FITC conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RG-16, monoclonal
• form: buffered aqueous solution
• should not react with: human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep
• storage condition: protect from light
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20, indirect immunofluorescence: 1:50 using using chicken fibroblast cells and primary antibody produced in rabbit
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Monoclonal Anti-Rabbit Immunoglobulins-FITC antibody produced in mouse has been used in:

• immunocytochemistry
• immunohistochemistry
• immunofluorescence

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

Physical form

Solution in 0.01 M sodium phosphate buffer, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)