F3679

Anti-Frizzled-7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-FZD7
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD06798447
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC (p)
• Citations: 5

Eigenschaften

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human
• availability: not available in Japan
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 4 μg/mL using human kidney
• UniProt accession no.: O75084
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... FZD7(8324) ; mouse ... Fzd7(14369)

Beschreibung

General description

Frizzled homolog protein (FZD) is a seven-pass transmembrane type receptor expressed in various normal tissues such as skeletal muscle, kidney, pancreas, cerebellum and cerebral cortex. In human, ten members (FZD1-FZD10) of Frizzled homolog protein have been identified. FZD7 is expressed throughout normal gastrointestinal tract, from esophagus to rectum. It is highly expressed in the gastric cancer cell line MKN7 and relatively lowers in esophageal cancer cell lines.

Immunogen

synthetic peptide corresponding to the N-terminal extracellular domain of human frizzled-7, conjugated to KLH. The immunizing peptide has 100% homology with the mouse gene.

Application

Anti-Frizzled-7 antibody is suitable for immunostaining of liver tissues to localise FZD7 in T and pT tissues. It is also suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections) at a concentration of 4μg/mL using human kidney.

Biochem/physiol Actions

FZD7 shows 98.8% nucleotide identity with FzE3 and encodes for WNT receptors with nine amino-acid substitutions. In human primary gastric cancer, high level of FZD7 has been found. Mechanistically, over-expression of Frizzled-7 accelerates the WNT-β-catenin-TCF pathway. Through the activation of the WNT-β-catenin-TCF pathway, FZD7, might play key roles in carcinogenesis.

Physical form

Solution in phosphate buffered saline containing 0.01% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids