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E9640

Sigma-Aldrich

Endothelial cell growth factor

100 ×, sterile-filtered, suitable for cell culture

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About This Item

UNSPSC-Code:
12352202

Biologische Quelle

bovine pituitary glands

Qualitätsniveau

200

Sterilität

sterile-filtered

Verpackung

pkg of 1 L
pkg of 10 mL
pkg of 5 mL

Lagerbedingungen

avoid repeated freeze/thaw cycles

Konzentration

100 ×

Methode(n)

cell culture | mammalian: suitable

Verunreinigungen

endotoxin, tested

Versandbedingung

dry ice

Lagertemp.

−70°C

Anwendung

Endothelial Cell Growth Factor (ECGF) has been optimized for the culture of Human Umbilical Vein Endothelial Cells (HUVEC). ECGF is optimized for the mitogenic proliferation of HUVEC cells when supplemented to CS-C Medium. ECGF should be supplemented to CS-C medium at a concentration 1 ml per 100 ml of culture medium. ECGF is prepared in DPBS (without calcium and magnesium) supplemented with porcine heparin and HBGF-1. For best results it is recommended that this product be used in conjugation with CS-C Medium for Endothelial Cell Lines (Product Nos. C 1431 or C 1556), Endothelial Attachment Factor (Product No. E 9765), Trypsin/EDTA Solution for Endothelial Cell Cultures (Product No. T 4299) and Trypsin Inhibitor Solution (Product No. T 6414).

Angaben zur Herstellung

5 mg/ml each porcine heparin and HBGF-1 in DPBS without calcium and magnesium

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

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