E8034

Monoclonal Anti-EEA1 antibody produced in mouse

~1.0 mg/mL, clone EEA1-C33, purified immunoglobulin, buffered aqueous solution

• Synonym(e): Anti-Early endosomal antigen 1, Anti-MST105, Anti-MSTP105, Anti-ZFYVE2
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: EEA1-C33, monoclonal
• Application: WB
• Citations: 10

Eigenschaften

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: EEA1-C33, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~160 kDa
• species reactivity: human, mouse, rat
• concentration: ~1.0 mg/mL
• technique(s): western blot: 2-4 μg/mL using whole extract of rat NRK or human HeLa cells
• isotype: IgG1
• UniProt accession no.: Q15075
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... EEA1(8411) ; mouse ... Eea1(216238) ; rat ... Eea1(314764)

Beschreibung

General description

The gene Early Endosome Antigen 1 (EEA1) encodes for around 1400 amino acid proteins. It is a peripheral membrane protein associated with the cytoplasmic face of early endosomes. EEA1 is a autoantigen associated with subacute cutaneous systemic lupus erythematosus and is a coiled-coil protein localized to early endosomes and cytosol.

Application

Monoclonal Anti-EEA1 antibody produced in mouse is suitable for the following applications:

• Western blotting (at a concentration of 2-4μg/mL using whole extract of rat NRK or human HeLa cells)
• Immunofluorescence Analysis
• Immunocytochemistry

Biochem/physiol Actions

Early Endosome Antigen 1 (EEA1) controls vesicle fusion during endocytosis. In neurons, it is involved in recycling of synaptic vesicles and neurotransmitter receptors. Patients with neurological deficits develop EEA1 autoantibodies. Mutation in EEA1 gene leads to susceptibility to diabetes in the Japanese population. Phosphatidylinositol 3-phosphate (PtdIns(3)P) is essential for the localization and function of EEA1.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.