E5389

Erythromycin

powder, suitable for cell culture, BioReagent

• Synonym(e): E-Mycin, Erythrocin
• Empirische Formel (Hill-System): C₃₇H₆₇NO₁₃
• CAS-Nummer: 114-07-8
• Molekulargewicht: 733.93
• NACRES: NA.76
• PubChem Substance ID: 24894612
• UNSPSC Code: 12352207
• EC Number: 204-040-1
• MDL number: MFCD00084654
• Beilstein/REAXYS Number: 75279

Eigenschaften

• Produktname: Erythromycin, BioReagent, suitable for cell culture
• product line: BioReagent
• Quality Segment: 200
• form: powder
• potency: ≥850 μg per mg
• technique(s): cell culture | mammalian: suitable
• impurities: ≤0.1 EU/mg endotoxin
• color: white
• mp: 133 °C
• solubility: 2 M HCl: 50 mg/mL (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.), ethanol: soluble (Stock solutions should be stored at 2-8 °C. Stable at 37 °C for 3 days.)
• antibiotic activity spectrum: Gram-negative bacteria, Gram-positive bacteria
• mode of action: protein synthesis | interferes
• SMILES string: CC[C@H]1OC(=O)[C@H](C)[C@@H](O[C@H]2C[C@@](C)(OC)[C@@H](O)[C@H](C)O2)[C@H](C)[C@@H](O[C@@H]3O[C@H](C)C[C@@H]([C@H]3O)N(C)C)[C@](C)(O)C[C@@H](C)C(=O)[C@H](C)[C@@H](O)[C@]1(C)O
• InChI: 1S/C37H67NO13/c1-14-25-37(10,45)30(41)20(4)27(39)18(2)16-35(8,44)32(51-34-28(40)24(38(11)12)15-19(3)47-34)21(5)29(22(6)33(43)49-25)50-26-17-36(9,46-13)31(42)23(7)48-26/h18-26,28-32,34,40-42,44-45H,14-17H2,1-13H3/t18-,19-,20+,21+,22-,23+,24+,25-,26+,28-,29+,30-,31+,32-,34+,35-,36-,37-/m1/s1
• InChI key: ULGZDMOVFRHVEP-RWJQBGPGSA-N
• Gene Information: human ... ABCB1(5243), CYP3A4(1576), MLNR(2862) ; mouse ... Abcb1a(18671), Abcb1b(18669)

Beschreibung

General description

Chemical structure: macrolide

Erythromycin is an antibiotic produced by growth of certain strains of Streptomyces erythreus. This product is composed largely of erythromycin A with small amounts of erythromycins B and C and is recommended for concentration at 100 mg/L. Concentrations between 50 and 200 mg/L have also proven effective in controlling bacterial growth. Erythromycin has been used as a motilin receptor agonist, to block respiratory glycoconjugate secretion in human airways in vitro, and for selecting plasmid-cured and recombinant lactococcus lactis MG1363 strains.

Application

Erythromycin has been used:

• as a supplement for nutrient broth medium for culturing green fluorescent protein (GFP)- expressing E. coli
• as a model drug to determine small intestinal (SMI) microtissue viability using the MTT assay{254
• as an antibiotic to study the treatment strategies of chronic infections

Biochem/physiol Actions

Wirkungsweise: Inhibiert Elongation bei dem Transpepditierungsschritt.
Antimikrobielles Spektrum: Gram-negative und Gram-positive Bakterien.

Preparation Note

This product is soluble in water at 2 mg/mL, with a 0.067% solution in water yielding a pH of 8.0-10.5. It is also soluble in ethanol at 50 mg/mL, yielding a clear, colorless to faint yellow solution. It is freely soluble in alcohol, acetone, chloroform, acetonitrile and ethyl acetate but forms salts with acids. All solutions should be protected from light.

Disclaimer

This product is stable in solution at 37°C for 3 days. Stock solutions should be stored at 2-8°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)