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E0632

Enterokinase from porcine intestine

≥0.5 units/mg solid

Synonym(e):

Enteropeptidase

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Ihnen/SKUVerfügbarkeitPreis
1500 units
Warenkorb auf Verfügbarkeit prüfen
€ 389,00

Über diesen Artikel

CAS-Nummer:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-761-1
NACRES:
NA.54
MDL number:
EG-Nummer:
Specific activity:
≥0.5 units/mg solid

€ 389,00


Warenkorb auf Verfügbarkeit prüfen

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form

salt-free, lyophilized powder

Quality Segment

specific activity

≥0.5 units/mg solid

mol wt

150 kDa

storage temp.

−20°C

Application

Enterokinase from porcine intestine has been used in a study to report a new experimental model of the anomalous pancreatico-biliary junction. Enterokinase from porcine intestine has also been used in a study to investigate the insulinotropic region of the gastric inhibitory polypeptide.
The enzyme from Sigma has been used for the activation of trypsinogen in order to measure the activity of trypsin in hog pancreas. The study showed that antimicrobial treatment reduces intestinal microflora and improves protein digestive capacity without changes in villous structure of weanling pigs.[1]

Biochem/physiol Actions

Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination.

Other Notes

One unit will produce 1.0 nanomole of trypsin from trypsinogen per min at pH 5.6 at 25 °C.

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Dieser Artikel
E0885E5144A8376
specific activity

≥0.5 units/mg solid

specific activity

≥100 units/mg protein

specific activity

-

specific activity

300-1,500 units/mg protein

form

salt-free, lyophilized powder

form

lyophilized powder

form

powder

form

salt-free, lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

150 kDa

mol wt

150 kDa

mol wt

150 kDa (consisting of 115kDa and 35kDa subunits.)

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200


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Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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