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Merck

CG200

Sigma-Aldrich

cGMP Enzyme Immunoassay Kit, Direct

sufficient for 96 assays

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About This Item

UNSPSC-Code:
12161503
NACRES:
NA.84

Verwendung

sufficient for 96 assays

Qualitätsniveau

Methode(n)

ELISA: suitable

Versandbedingung

wet ice

Lagertemp.

−20°C

Angaben zum Gen

Anwendung

Non-radioactive, competitive immunoassay for the quantitation of total cGMP in tissue and cell cultures. Buffer contains 0.1N HCl that aids in the lysis of cells, inhibits endogenous phosphodiesterases and stabilizes the cyclic nucleotides. This kit uses a polyclonal antibody to cGMP to competitively bind cGMP or cGMP that has been covalently linked to an alkaline phosphatase molecule. The assay is performed in a 96 well plate coated with anti-rabbit IgG antibody. The colored end product, produced by the addition of substrate to the wells, is read at 405 nm on a multiwell plate reader. The intensity of the color is inversely proportional to the concentration of cGMP present in the well.
Please refer to the attached Protocolfor details.

Nur Kit-Komponenten

Produkt-Nr.
Beschreibung

  • 0.1 M Hydrochloride Acid 1 x 30

  • Acetic Anhydride 1 x 1

  • cGMP-Alkaline Phosphatase Conjugate 1 x 5

  • cGMP EIA Antibody Rabbit Anti-cGMP 1 x 5

  • Cyclic GMP Standard 1 x 0.5

  • Goat Anti-Rabbit IgG Coated 96 Well Microtiter Plate 1 ea

  • Neutralizing Reagent 1 x 6

  • p-Nitrophenyl Phosphate Substrate Solution 1 x 20

  • Plate Sealer 1 ea

  • Stop Solution 1 x 5

  • Triethylamine 1 x 2

  • Wash Buffer Concentrate 1 x 30

Alle anzeigen (12)

Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3

Zielorgane

Respiratory system

Zusätzliche Gefahrenhinweise

Lagerklassenschlüssel

3 - Flammable liquids

Flammpunkt (°F)

120.2 °F

Flammpunkt (°C)

49 °C


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