3T3 clone A31 Cell Line from mouse

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86110401, mouse embryo, Fibroblast

• Synonym(e): 3T3-A31 Cells, BALB/3T3 clone A31 Cells, BALB/c 3T3 clone A31 Cells
• UNSPSC Code: 41106514
• Biological source: mouse embryo
• Growth mode: Adherent
• Karyotype: 2n = 40, hypotetraploid
• Morphology: Fibroblast

Eigenschaften

• biological source: mouse embryo
• description: Mouse BALB/c embryo
• packaging: tube of 5 μg 86110401-DNA-5UG, pkg of vial of cells 86110401-1VL
• growth mode: Adherent
• karyotype: 2n = 40, hypotetraploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity and virus studies: SV40 and MSV

3T3 clone A31 cell line from mouse has been used:

• to study in vitro cytotoxicity and genotoxicity of retinyl palmitate–loaded poly(lactide-co-glycolide) nanoparticles for the topical treatment of skin diseases
• for the characterization of the phototoxicity of Vemurafenib, which is a B-Raf serine/threonine kinase inhibitor
• for the phototoxicity assessment of systemically applied compounds and drug candidates

Biochem/physiol Actions

Cells have now lost their contact inhibition.

Mouse BALB/c embryo

Preparation Note

DMEM + 2mM Glutamine + 5% New Born Calf Serum (NBCS) + 5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000 to 2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].