RD

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85111502, human embryo, Spindle shaped

• UNSPSC Code: 41106514
• Biological source: human embryo
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Modal no. 56
• Morphology: Spindle shaped

Eigenschaften

• biological source: human embryo
• packaging: tube of 5 μg 85111502-DNA-5UG, pkg of vial of cells 85111502-1VL
• growth mode: Adherent
• karyotype: Modal no. 56
• morphology: Spindle shaped
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Beschreibung

Application

Tumourigenicity studies

Biochem/physiol Actions

Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female. Also designated TE 32 and 130T in the literature

Human Caucasian embryo rhabdomyosarcoma

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 9
vWA: 18

Preparation Note

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to [email protected].