C0618

Anti-Calsequestrin-1

~1.6 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-CASQ1, Aspartactin, CALMITINE, Calsequestrin- skeletal muscle isoform
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD09265355
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 9

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen ~60 kDa
• species reactivity: canine, mouse, human, rat
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• concentration: ~1.6 mg/mL
• technique(s): western blot: 0.5-1 μg/mL using extracts of rat skeletal muscle S1 fraction
• UniProt accession no.: P31415
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CASQ1(844) ; mouse ... Casq1(12372)

Beschreibung

General description

Calsequestrin (CS also known as CSQ), the major Ca²⁺ binding protein in cardiac and skeletal muscle, is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which function as an internal Ca²⁺ store in the lumen of the SR.1-3 In mammals, two forms of the protein exist, calsequestrin-1 (CASQ-1) and calsequestrin-2 (CASQ-2), which encode the fast-twitch skeletal muscle and cardiac calsequestrin, respectively. Calsequestrin-1 is found in the SRs terminal cisternae luminal space of fast skeletal muscle cells. Calsequestrin-1 is a putative autoantigen associated with eye muscle inflammation in Graves′ disease.

Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca²⁺ release with the help of triadin and junction proteins.

Immunogen

synthetic peptide corresponding to amino acids 66-84 located near the N-terminus of mouse calsequestrin-1, conjugated to KLH. This sequence is identical in human, rat, and dog calsequestrin-1 and highly conserved in bovine (single amino acid substitution). This sequence is not found in calsequestrin-2.

Application

Anti-Calsequestrin-1 antibody produced in rabbit has been used in immunofluorescence.

Anti-Calsequestrin-1 antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using extracts of rat skeletal muscle S1 fraction.

Biochem/physiol Actions

Calsequestrin-1 is a putative auto-antigen associated with eye muscle inflammation in Graves′ disease. Calsequestrin-2 (55 kDa) is found in the SR′s terminal cisternae luminal space of cardiac, slow skeletal muscle cells and may be in platelets. The major Ca²⁺ binding protein in cardiac and skeletal muscle is a high-capacity, low-affinity Ca²⁺ binding glycoprotein, which functions as an internal Ca²⁺ store in the lumen of the SR. It helps in controlling the smooth-muscle contraction. Calsequestrin is expected to modulate ryanodine receptor (RyR) signaling and Ca²⁺ release with the help of triadin and junction proteins.†

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)