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Merck

B9754

BIS-TRIS

≥98.0% (titration)

BIS-TRIS

Synonym(e):

(2,2-Bis-hydroxymethyl)-2,2′,2″-nitrilotriethanol, 2-Bis-(2-hydroxyethyl)-amino-2-(hydroxymethyl)-1,3-propandiol, Bis-(2-hydroxyethyl)-amino-tris-(hydroxymethyl)-methan

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Über diesen Artikel

Empirische Formel (Hill-System):
C8H19NO5
CAS-Nummer:
6976-37-0
Molekulargewicht:
209.24
UNSPSC Code:
12161700
NACRES:
NA.25
PubChem Substance ID:
24892155
EC Number:
230-237-7
Beilstein/REAXYS Number:
2205275
MDL number:
MFCD00002853

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Unterstützung erhalten

assay

≥98.0% (titration)

form

crystalline powder

storage condition

dry at room temperature

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

color

white

useful pH range

5.8-7.2

pKa (25 °C)

6.5

solubility

H2O: 500 mg/mL, clear, colorless to very faintly yellow

suitability

suitable for Western blot, suitable for column chromatography of proteins

application(s)

diagnostic assay manufacturing
general analytical
microbiology

SMILES string

OCCN(CCO)C(CO)(CO)CO

InChI

1S/C8H19NO5/c10-3-1-9(2-4-11)8(5-12,6-13)7-14/h10-14H,1-7H2

InChI key

OWMVSZAMULFTJU-UHFFFAOYSA-N

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Dieser Artikel
B7535B4429391335
BIS-TRIS ≥98.0% (titration)

B9754

BIS-TRIS

BIS-TRIS BioXtra, ≥98.0% (titration)

B7535

BIS-TRIS

BIS-TRIS BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, ≥98.0%

B4429

BIS-TRIS

BIS-TRIS ULTROL® Grade, ≥99% dry basis (titration), suitable where a calcium phosphate-DNA complex formation is desirable

Millipore

391335

BIS-TRIS

assay

≥98.0% (titration)

assay

≥98.0% (titration)

assay

≥98.0%

assay

≥99% dry basis (titration)

technique(s)

UV/Vis spectroscopy: suitable, electrophoresis: suitable

technique(s)

-

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable

technique(s)

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

application(s)

diagnostic assay manufacturing
general analytical
microbiology

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

form

crystalline powder

form

powder

form

crystalline powder

form

solid

storage condition

dry at room temperature

storage condition

-

storage condition

-

storage condition

OK to freeze

General description

Bis-Tris-Methan, auch bekannt als BIS-TRIS oder BTM, ist ein zwitterionisches Puffermittel, das in der biochemischen und biologischen Forschung weit verbreitet ist. Es funktioniert wirksam in wässrigen Lösungen mit einem pH-Bereich von 5,8 bis 7,2 und weist minimale Verschiebungen in der Dissoziationskonstante auf. Darüber hinaus kann es als Ersatz für den hochtoxischen Puffer Cacodylat verwendet werden und ist auch für seine Komplexbildung mit verschiedenen Metallen bekannt.

Application

Bis-Tris wird wie folgt eingesetzt:
  • als Puffer für die Elektrophorese[1]
  • als Komponente des Transferpuffers für Western Blot[2]

Features and Benefits

  • Geeignet für die Forschung in den Bereichen der Biologie und Biochemie
  • Effektive Pufferung im pH-Bereich 5,8 bis 7,2 (25 °C) mit einem pKa-Wert von 6,5 (25 °C)

Other Notes

Füllen Sie bitte dieses Formular aus, um weitere Informationen zu unserem Sortiment an Biochemikalien zu erhalten.

Comparable product

Produkt-Nr.
Beschreibung
Preisangaben

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H319

Hazard Classifications

Eye Irrit. 2

Lagerklasse

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000540579
0000535222
0000535224
0000532390
0000532389

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Besitzen Sie dieses Produkt bereits?

In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.

Die Dokumentenbibliothek aufrufen

Raman Kumar et al.
STAR protocols, 3(4), 101693-101693 (2022-09-20)
Immunoprecipitation (IP) of endogenously expressed proteins is one of the most biologically relevant techniques to identify protein-protein interactions. We describe an adaptable IP protocol reliant on a specific antibody to the target protein. We detail a quantitative proteomics workflow for
Zahra Sabouri et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(25), E2567-E2575 (2014-05-14)
The best-understood mechanisms for achieving antibody self/non-self discrimination discard self-reactive antibodies before they can be tested for binding microbial antigens, potentially creating holes in the repertoire. Here we provide evidence for a complementary mechanism: retaining autoantibodies in the repertoire displayed
Alastair W Skeffington et al.
Plant physiology, 165(2), 866-879 (2014-05-02)
The first step on the pathway of starch degradation in Arabidopsis (Arabidopsis thaliana) leaves at night is the phosphorylation of starch polymers, catalyzed by glucan, water dikinase (GWD). It has been suggested that GWD is important for the control of
Elizabeth M MacDonald et al.
Disease models & mechanisms, 7(4), 471-481 (2014-02-08)
The purpose of our study was to compare two acquired muscle atrophies and the use of myostatin inhibition for their treatment. Myostatin naturally inhibits skeletal muscle growth by binding to ActRIIB, a receptor on the cell surface of myofibers. Because
Lesley Cheng et al.
Kidney international, 86(2), 433-444 (2013-12-20)
Micro RNAs (miRNAs) have been shown to circulate in biological fluids and are enclosed in vesicles such as exosomes; they are present in urine and represent a noninvasive methodology to detect biomarkers for diagnostic testing. The low abundance of RNA
Alle zugehörigen Dokumente anzeigen

Protokolle

Enzymatic Activity of Glucose-6-Phosphatase [EC 3.1.3.9]

To measure glucose-6-phosphatase activity, the Taussky-Shorr method is used. This method is a spectrophotometric stop-rate determination assay that is measured at 660 nm.

Verwandter Inhalt

Product Information Sheet - B9754

Product Information Sheet

Global Trade Item Number

SKUGTIN
B9754-1KG04061833443941
B9754-500G04061833443965
B9754-100G04061833443934
B9754-25G04061833443958
B9754-25KG04061832546629
B9754-5KG04061833443972
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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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