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B8291

BSK-H Medium, Complete

With 6% rabbit serum, liquid, sterile-filtered, suitable for cell culture

Synonym(e):

Barbour-Stoenner-Kelly medium, Borrelia burgdorferi medium

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PackungsgrößeSKUVerfügbarkeitPreis
100 mL
Warenkorb auf Verfügbarkeit prüfen
€ 92,30
500 mL
Warenkorb auf Verfügbarkeit prüfen
€ 269,00
1 L
Warenkorb auf Verfügbarkeit prüfen
€ 462,00

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.75

€ 92,30


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Produktname

BSK-H Medium, Complete, sterile-filtered, with 6% rabbit serum, suitable for Borrelia burgdorferi

Quality Segment

sterility

sterile-filtered

form

liquid

suitability

suitable for Borrelia burgdorferi

application(s)

food and beverages

components

NaHCO3: 2.068 g/L
phenol red: 0.01997 g/L
glucose: 5.64 g/L (Dextro)
HEPES: 5.64 g/L

shipped in

dry ice

storage temp.

−20°C

Application

Complete BSK-H Medium was used to culture B. burgdorferi to study the pathology of lyme borreliosis.[1][2] It may be used in serum supplemented formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi.

Biochem/physiol Actions

BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.[3]

Preparation Note

BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.

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Dieser Artikel
B3528S014651417C
suitability

suitable for Borrelia burgdorferi

suitability

suitable for Borrelia burgdorferi (Qualified)

suitability

-

suitability

-

sterility

sterile-filtered

sterility

sterile; sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

application(s)

food and beverages

application(s)

food and beverages

application(s)

-

application(s)

-

components

NaHCO3: 2.068 g/L
phenol red: 0.01997 g/L
glucose: 5.64 g/L (Dextro)
HEPES: 5.64 g/L

components

NaHCO3: 2.2 g/L
glucose: 6.0 g/L (Dextro)
phenol red: 0.02124 g/L
HEPES: 6.0 g/L

components

NaHCO3: 0.4 g/L
glucose: 2 g/L (Dextro)
L-glutamine: 1.8 g/L

components

L-glutamine: 292 mg/L
phenol red: 10.62 mg/L
Earle’s salts (5% CO2): yes
NaHCO3: 840 mg/L
HEPES: 4766.4 mg/L

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C


Lagerklasse

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

64-17-5

CAS No.


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Ghiabe H Guibinga et al.
Human vaccines & immunotherapeutics, 16(9), 2114-2122 (2020-08-14)
Lyme disease is the most common vector-borne disease in North America. The etiological agent is the spirochete Borreliella burgdorferi, transmitted to mammalian hosts by the Ixodes tick. In recent years there has been an increase in the number of cases
Deborah A Grosenbaugh et al.
BMC veterinary research, 14(1), 312-312 (2018-10-18)
Prevention of Lyme disease in dogs in North America depends on effective vaccination against infection by the tick vector-born spirochete Borrelia burgdorferi. Most vaccines effectively prevent spirochete transmission to dogs during tick feeding based on immunization with the outer-surface lipoprotein
Sin Hang Lee et al.
American journal of clinical pathology, 133(4), 569-576 (2010-03-17)
The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature



Global Trade Item Number

SKUGTIN
B8291-500ML04061833442685
B8291-100ML04061833442661
B8291-1L04061833442678

Questions

  1. Is rabbit serum inactivated by heat? Thanks

    1 answer
    1. No, this product has not been heat inactivated.

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