B6149

5-Brom-4-Chlor-3-Indolylphosphat Dinatriumsalz

chromogenic, ≥98% (HPLC), powder

• Synonym(e): BCIP^®, X-phosphat Dinatriumsalz
• Lineare Formel: C₈H₄BrClNO₄P · 2Na
• CAS-Nummer: 102185-33-1
• Molekulargewicht: 370.43
• MDL-Nummer: MFCD00036757
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24891897
• NACRES: NA.83

Eigenschaften

• product name: 5-Brom-4-Chlor-3-Indolylphosphat Dinatriumsalz, ≥98% (HPLC)
• Assay: ≥98% (HPLC)
• Form: powder
• Löslichkeit: H₂O: 20 mg/mL; DMF: insoluble
• Lagertemp.: −20°C
• SMILES String: [Na+].[Na+].[O-]P([O-])(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12
• InChI: 1S/C8H6BrClNO4P.2Na/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;/q;2*+1/p-2
• InChIKey: OAZUOCJOEUNDEK-UHFFFAOYSA-L

Beschreibung

Anwendung

For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry.

BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid.

5-Bromo-4-chloro-3-indolyl phosphate disodium salt has been used to detect protein-antibody complexes. It has also been used for tissue staining in alkaline phosphatase histochemistry.

Substrate

Histochemisches Substrat für alkaline Phosphatase.

Rechtliche Hinweise

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)