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HighPrep PCR Clean-up System

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Über diesen Artikel

UNSPSC Code:
41105500


form

liquid

packaging

pkg of 250 mL (AC-60250 (HighPrep PCR (250 mL))), pkg of 5 mL (AC-60005 (HighPrep PCR (5 mL))), pkg of 50 mL (AC-60050 (HighPrep PCR (50 mL))), pkg of 500 mL (AC-60500 (HighPrep PCR (500 mL)))

storage condition

do not freeze

storage temp.

2-8°C

General description

Magnetic beads-based reagent for manual and for automated post-PCR and post-enzymatic reaction clean-up, for NGS library clean-up and amplicon size selection.

The HighPrep PCR Clean-up System is a paramagnetic beads-based post PCR clean-up reagent designed for efficient purification of DNA fragments and for size-specific selection of amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic bead particles. Highly purified DNA is eluted with low salt elution buffer or water which can be used directly for downstream applications, such as library construction, next generation sequencing (NGS), Sanger sequencing, cloning, restriction digestions, adapoter ligation, microarrays, and so on. The magnetic beads are used for different library prep chemistries for NGS.

Application

HighPrep PCR Clean-up System may be used in:
  • Post-PCR and post-enzymatic reaction clean-up
  • NGS library preparation
  • Amplicon size selection for NGS
  • Microarrays
  • PCR
  • Sanger sequencing
  • Restriction enzyme digestions, adapter ligations
  • Cloning

Features and Benefits

  • High recovery of >100 bp amplicons
  • Adaptable to high throughput liquid handling systems
  • Stable recovery of amplicons greater than 100bp, predictable and consistent size selection
  • No centrifugation or filtration leads to higher and purer yields

Preparation Note

14 months when stored as directed

Legal Information

HighPrep is a trademark of MagBio Genomics, Inc.

Disclaimer

For Research Use Only. Not for Use in Diagnostic Procedures.


pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Repr. 2

Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Protokolle

HighPrep™ PCR purification magnetic clean-up beads for manual and automated post-PCR, post-enzymatic reaction, NGS library clean-up.






Global Trade Item Number

SKUGTIN
AC-6000504061841079965
AC-6050004061841079996
AC-6025004061841079989
AC-6005004061841079972

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