A8076

Monoklonales Anti-Phosphoserin

clone PSR-45, purified from hybridoma cell culture

• Synonym(e): Phospho Ser, Phospho−Ser, Phospho−serin, Phospho-serin
• NACRES: NA.44
• UNSPSC Code: 12352203
• Conjugate: agarose conjugate
• Clone: PSR-45, monoclonal
• Application: IP
• Citations: 7

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: agarose conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: PSR-45, monoclonal
• form: buffered aqueous solution
• technique(s): immunoprecipitation (IP): suitable
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

Monoclonal Anti-Phosphoserine (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Immunogen

Phosphoserin konjugiert zu Keyhole Limpet-Hämocyanin (KLH).

Application

Monoclonal Anti-Phosphoserine - Agarose may be used for the immunoprecipitation or immuno-affinity purification of serine phosphoproteins.The antibody may be used for the localization of some phosphoserine containing proteins using the immunoblotting method.

Biochem/physiol Actions

Bei ELISA und Dot Blot reagiert der Antikörper spezifisch mit phosphoryliertem Serin, sowohl als freie Aminosäure als auch konjugiert zu Trägern wie BSA oder KLH. Es wurde keine Kreuzreaktivität mit nicht phosphoryliertem Serin, Phosphothreonin, Phosphotyrosin, AMP oder ATP beobachtet. Dieser Antikörper wurde in Immunoblotting zur Lokalisation einiger Phosphoserin-haltiger Proteine eingesetzt. Bestimmte Proteine, die bekanntermaßen phosphoryliertes Serin enthalten, werden durch diesen Antikörper möglicherweise nicht erkannt, da die Erkennungsstelle sterisch behindert wird.

Reversible phosphorylation of proteins is an important post-translational modification that plays a regulatory role in the expression of most proteins in the cells. Reversible phosphorylation at multiple serine, tyrosine and threonine residues mediate numerous signalling pathways in both prokaryotic and Eukaryotic cells. Cellular proteins with phosphorylated serine increase many fold by the activation of serine kinases. Most mitogenic receptor systems such as EGF, PDGF, insulin receptors contain serine/threonine/tyrosine kinase domains that undergo autophosphorylation when receptors bind to the respective ligands. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases,3 and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.
Monoclonal Anti-Phosphoserine may be used for the identification of proteins containing phosphorylated serine both as the free amino acid or when conjugated to carriers such as BSA or KLH. It does not react with non-phosphorylated serine, phosphorylated tyrosine or threonine, AMP or ATP.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable