A5670

Anti-Pig IgG (whole molecule)−Peroxidase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(e): Anti-Pig Antibodies, Anti-Pig Antibodies - Anti-Pig IgG (whole molecule)-Peroxidase antibody produced in rabbit, Anti-Porcine IgG (whole molecule)−Peroxidase antibody produced in rabbit, Anti-Swine IgG (whole molecule)−Peroxidase antibody produced in rabbit
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162752
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d)
• Citations: 22

Eigenschaften

• biological source: rabbit
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: pig
• technique(s): direct ELISA: 1:40,000, dot blot: 1:150,000 (chemiluminescent)
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Beschreibung

General description

Immunoglobulin is a glycoprotein, with two heavy chain and two light chain connected by disulfide bond. IgG is a major class of immunoglobulin. It has five subclasses of IgG- IgG1, IgG3, IgG2a and IgG2b. Affinity isolated antibody is obtained from rabbit anti-pig IgG antiserum by immunospecific purification which removes essentially all rabbit serum proteins, including immunoglobulins, which do not specifically bind to pig IgG. Anti-Pig IgG is then conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde.

Immunogen

purified pig IgG

Application

Anti-Pig IgG (whole molecule)?Peroxidase antibody produced in rabbit has been used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III. It mainly helps in immune defense. Pig IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary anti-pig IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Rabbit anti-pig IgG (whole molecule)-peroxidase antibody binds to pig IgG.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable