A4541
Anti-Biotin−Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Synonym(e):
Anti-Biotin, Anti Biotin Hrp
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0.25 ML
€ 131,00
0.5 ML
€ 172,00
1 ML
€ 309,00
Über diesen Artikel
NACRES:
NA.46
UNSPSC Code:
12352203
Conjugate:
peroxidase conjugate
Clone:
polyclonal
Application:
ELISA (d)
Citations:
10
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Unterstützung erhaltenbiological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
technique(s)
direct ELISA: 1:2,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Verwandte Kategorien
1 of 4
Dieser Artikel | |||
|---|---|---|---|
| conjugate peroxidase conjugate | conjugate alkaline phosphatase conjugate | conjugate unconjugated | conjugate agarose conjugate |
| biological source goat | biological source goat | biological source goat | biological source mouse |
| antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form purified immunoglobulin |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| clone polyclonal | clone polyclonal | clone polyclonal | clone BN-34, monoclonal |
| form buffered aqueous solution | form buffered aqueous glycerol solution | form lyophilized powder | form PBS suspension |
General description
Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. Goat anti-biotin-peroxidase antibody can be used in many applications where biotin may be introduced as target label.
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Immunogen
Biotin-KLH conjugate
Application
Biotin-labeled proteins in U937 and BHK cell lysates were analyzed by western blot using HRP-conjugated goat anti-biotin.
Goat anti-biotin-peroxidase antibody can be used for direct ELISA assays at a dilution of 1:2,000.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.
May be substituted for avidin to decrease non-specific binding.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin with preservative.
Preparation Note
Prepared by the periodate method described by Wilson, M.B., and Nakane, P.K., in "Immunofluorescence and Related Staining Techniques," Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
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Samuel H Henager et al.
Nature methods, 13(11), 925-927 (2016-11-01)
Expressed protein ligation is a valuable method for protein semisynthesis that involves the reaction of recombinant protein C-terminal thioesters with N-terminal cysteine (N-Cys)-containing peptides, but the requirement of a Cys residue at the ligation junction can limit the utility of
Katarzyna Kwiatkowska et al.
Journal of cell science, 116(Pt 3), 537-550 (2003-01-01)
Activation of Fcgamma receptor II (FcgammaRII) induces rearrangement of the actin-based cytoskeleton that serves as a driving force for FcgammaRII-mediated phagocytosis and FcgammaRII capping. To get insight into the signaling events that lead to the actin reorganization we investigated the
Soledad Astrada et al.
Journal of peptide science : an official publication of the European Peptide Society, 22(11-12), 711-722 (2016-12-10)
Because of resistance development by cancer cells against current anticancer drugs, there is a considerable interest in developing novel antitumor agents. We have previously demonstrated that CIGB-552, a novel cell-penetrating synthetic peptide, was effective in reducing tumor size and increasing
Nataliya Gorinski et al.
Nature communications, 10(1), 3924-3924 (2019-09-04)
The serotonergic system and in particular serotonin 1A receptor (5-HT1AR) are implicated in major depressive disorder (MDD). Here we demonstrated that 5-HT1AR is palmitoylated in human and rodent brains, and identified ZDHHC21 as a major palmitoyl acyltransferase, whose depletion reduced palmitoylation
Wei Zhou et al.
Cell death and differentiation, 28(11), 2991-3008 (2021-05-17)
Human death domain superfamily proteins (DDSPs) play important roles in many signaling pathways involved in cell death and inflammation. Disruption or constitutive activation of these DDSP interactions due to inherited gene mutations is closely related to immunodeficiency and/or autoinflammatory diseases;
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